DR5-Specific Polyclonal antibody

DR5-Specific Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB,ELISA

Conjugate

Unconjugated

Cat No : 15497-1-AP

Print datasheet

Synonyms

CD262, Death receptor 5, DR5, KILLER, KILLER/DR5, TNFRSF10B, TRAIL R2, TRAIL receptor 2, TRAILR2, TRICK2, TRICK2A, TRICK2B, TRICKB, ZTNFR9



经过测试的应用

Positive WB detected inHL-60 cells, PC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 11 publications below

产品信息

15497-1-AP targets DR5-Specific in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB,ELISA
Cited Applications WB
Tested Reactivity human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tumor necrosis factor receptor superfamily, member 10b
Synonyms CD262, Death receptor 5, DR5, KILLER, KILLER/DR5, TNFRSF10B, TRAIL R2, TRAIL receptor 2, TRAILR2, TRICK2, TRICK2A, TRICK2B, TRICKB, ZTNFR9
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 58-60 kDa, 45-50 kDa, 32 kDa
GenBank Accession NumberNM_003842
Gene Symbol TNFRSF10B
Gene ID (NCBI) 8795
RRIDAB_2240702
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14763
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TNFRSF10B, also known as CD262, DR5, TRAILR2, TRICK2 and KILLER, is a widely expressed single-pass type I membrane protein belonging to the tumour necrosis factor receptor superfamily (TNFRSF). It is a receptor for TNF-related apoptosis-inducing ligand (TRAIL), which is a member of the tumor necrosis factor (TNF) family of cytokines and induces apoptosis in a wide variety of cells (PMID: 9311998). TNFRSF10B contains two extracellular cysteine-rich repeats, typical for TNF receptor (TNFR) family members, and a cytoplasmic death domain (DD), through which TNFRSF10B is capable to transmit the apoptotic signal (PMID: 9311998; 20531300). In PDAC cells, especially Panc89 cells, it expresses additionally to the approximately 48 and 40 kDa forms of DR5, a faster migrating variant of DR5 of about 32 kDa(PMID:20354842). The TRAIL receptor 2 (death receptor 5, or DR5) forms receptor dimers in a ligand-dependent manner, and these receptor dimers exist within high molecular weight networks(Dr. Jonathan N. Sachs,2012).

实验方案

Product Specific Protocols
WB protocol for DR5-Specific antibody 15497-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

A non-canonical pathway regulates ER stress signaling and blocks ER stress-induced apoptosis and heart failure.

Authors - Yufeng Yao
humanWB

Int J Biol Sci

ESRG is critical to maintain the cell survival and self-renewal/pluripotency of hPSCs by collaborating with MCM2 to suppress p53 pathway

Authors - Shasha Li
humanWB

Biomed Pharmacother

Pro-survival effects by NF-κB, Akt and ERK(1/2) and anti-apoptosis actions by Six1 disrupt apoptotic functions of TRAIL-Dr4/5 pathway in ovarian cancer.

Authors - Juan Yang
humanWB

iScience

A small-molecule Skp1 inhibitor elicits cell death by p53-dependent mechanism.

Authors - Muzammal Hussain
humanWB

Mol Pharm

Discovery and characterization of 4-hydroxy-2-pyridone derivative sambutoxin as a potent and promising anticancer drug candidate: activity and molecular mechanism.

Authors - Lu-Ning Li
humanWB

Arch Biochem Biophys

Luteolin enhances TRAIL sensitivity in non-small cell lung cancer cells through increasing DR5 expression and Drp1-mediated mitochondrial fission.

Authors - Bin Wu