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CD30/TNFRSF8 Polyclonal antibody

CD30/TNFRSF8 Polyclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 55132-1-AP

Print datasheet

Synonyms

CD30, Ki-1 antigen, Ki 1 antigen, Ki 1, D1S166E

验证数据展示

  • IHC staining of human lymphoma using 55132-1-AP

    IHC staining of human lymphoma using 55132-1-AP

    Immunohistochemical analysis of paraffin-embedded human T-cell anaplastic large cell lymphoma (ALCL) tissue slide using 55132-1-AP (CD30 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lymphoma using 55132-1-AP

    IHC staining of human lymphoma using 55132-1-AP

    Immunohistochemical analysis of paraffin-embedded human T-cell anaplastic large cell lymphoma (ALCL) tissue slide using 55132-1-AP (CD30 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human lymphoma using 55132-1-AP

    IF Staining of human lymphoma using 55132-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human T-cell anaplastic large cell lymphoma (ALCL) tissue using CD30 antibody (55132-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of human lymphoma using 55132-1-AP

    IF Staining of human lymphoma using 55132-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human T-cell anaplastic large cell lymphoma (ALCL) tissue using CD30 antibody (55132-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inhuman lymphoma tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman lymphoma tissue

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55132-1-AP targets CD30/TNFRSF8 in IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IF-P, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tumor necrosis factor receptor superfamily, member 8
Synonyms CD30, Ki-1 antigen, Ki 1 antigen, Ki 1, D1S166E
Calculated Molecular Weight 64 kDa
GenBank Accession NumberNM_001243
Gene Symbol CD30
Gene ID (NCBI) 943
RRIDAB_2881274
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP28908
Storage Buffer 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD30 is a transmembrane glycoprotein belonging to the tumor necrosis factor receptor superfamily. CD30 expression is found on activated T and B cells. CD30 is useful in diagnosis of classic Hodgkin's lymphoma, anaplastic large cell lymphoma and embryonal carcinoma.

实验方案

Product Specific Protocols
IHC protocol for CD30/TNFRSF8 antibody 55132-1-APDownload protocol
IF protocol for CD30/TNFRSF8 antibody 55132-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols