TOP1 Polyclonal antibody

TOP1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 20705-1-AP

Print datasheet

Synonyms

DNA topoisomerase 1, DNA topoisomerase I, TOP1, TOPI, Top-I, TOPO1, topoisomerase (DNA) I



经过测试的应用

Positive WB detected inL02 cells
Positive IHC detected inhuman stomach cancer tissue, human colon cancer tissue, human cervical cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20705-1-AP targets TOP1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF, IHC, WB, ELISA
Cited ApplicationsIF, IHC, WB
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name topoisomerase (DNA) I
Synonyms DNA topoisomerase 1, DNA topoisomerase I, TOP1, TOPI, Top-I, TOPO1, topoisomerase (DNA) I
Calculated Molecular Weight 91 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberNM_003286
Gene Symbol TOP1
Gene ID (NCBI) 7150
RRIDAB_10695762
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP11387
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TOP1 belongs to the eukaryotic type I topoisomerase family. The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. The antibody is specific to TOP1.

实验方案

Product Specific Protocols
WB protocol for TOP1 antibody 20705-1-APDownload protocol
IHC protocol for TOP1 antibody 20705-1-APDownload protocol
IF protocol for TOP1 antibody 20705-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Nat Commun

Topoisomerase 1 cleavage complex enables pattern recognition and inflammation during senescence.

Authors - Bo Zhao
humanWB, IF

Cell Chem Biol

Lipid-derived electrophiles mediate the effects of chemotherapeutic topoisomerase I poisons.

Authors - Amy Flor
humanWB

J Transl Med

Integrated multi-omics analyses reveal Jorunnamycin A as a novel suppressor for muscle-invasive bladder cancer by targeting FASN and TOP1

Authors - Ruijiao Chen
humanWB

Oncotarget

Genetic variants in microRNAs predict non-small cell lung cancer prognosis in Chinese female population in a prospective cohort study.

Authors - Xia Lingzi
humanWB

J Cell Mol Med

EGCG synergizes the therapeutic effect of irinotecan through enhanced DNA damage in human colorectal cancer cells.

Authors - Wenbing Wu
mouseWB

iScience

Sensitization of ovarian tumor to immune checkpoint blockade by boosting senescence-associated secretory phenotype.

Authors - Xue Hao