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验证数据展示

  • WB analysis using 13987-1-AP

    WB analysis using 13987-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13987-1-AP (TRAK1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 13987-1-AP

    IHC staining of human stomach using 13987-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13987-1-AP (TRAK1 antibody) at dilution of 1:200 (under 10x lens)..

TRAK1 Polyclonal antibody

TRAK1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13987-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, ELISA

别名

Trafficking kinesin-binding protein 1, Protein Milton, OIP106, KIAA1042, 106 kDa O-GlcNAc transferase-interacting protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, MNK-45 cells
Positive IHC detected inhuman stomach tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:800
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

13987-1-AP targets TRAK1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性mouse
免疫原 TRAK1 fusion protein Ag5055 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 trafficking protein, kinesin binding 1
别名 Trafficking kinesin-binding protein 1, Protein Milton, OIP106, KIAA1042, 106 kDa O-GlcNAc transferase-interacting protein
计算分子量 953 aa, 106 kDa
观测分子量 64 kDa
GenBank蛋白编号BC015922
基因名称 TRAK1
Gene ID (NCBI) 22906
RRIDAB_2877999
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UPV9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TRAK1 antibody 13987-1-APDownload protocol
IHC protocol for TRAK1 antibody 13987-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Front Neurosci

O-GlcNAcylation is crucial for sympathetic neuron development, maintenance, functionality and contributes to peripheral neuropathy

Authors - Hsueh-Fu Wu
View Article