验证数据展示
经过测试的应用
| Positive WB detected in | Human brain, fetal tissue, human heart tissue |
| Positive IHC detected in | human liver tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | PC-12 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
66108-1-Ig targets Prealbumin/transthyretin in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, FC (Intra), ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | Prealbumin/transthyretin fusion protein Ag19232 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | transthyretin |
| Synonyms | Prealbumin, transthyretin, TTR, TBPA, PALB |
| Calculated Molecular Weight | 147 aa, 16 kDa |
| Observed Molecular Weight | 14-16 kDa, 28-33 kDa |
| GenBank Accession Number | BC020791 |
| Gene Symbol | TTR |
| Gene ID (NCBI) | 7276 |
| RRID | AB_2881507 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P02766 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Transthyretin (TTR) is a plasma transport protein for thyroxine and retinol, through the association with retinol-binding protein. It is a homotetrameric protein synthesized mainly in liver, choroid plexus, retinal pigment epithelium, and pancreas.Within the CNS, TTR is the only known protein synthesized solely by the choroid plexus. Mutant and wildtype TTR give rise to various forms of amyloid deposition (amyloidosis). Defects in TTR are the cause of amyloidosis transthyretin-related (AMYL-TTR),hyperthyroxinemia dystransthyretinemic euthyroidal (HTDE) and carpal tunnel syndrome type 1 (CTS1). In addition, positive immunostaining for TTR has been reported as a sensitive diagnostic marker of choroid plexus tumors. TTR can be expressed as monomer (14-16 kDa), dimmer (28-33 kDa), or tetramer around 55-60 kDa. (PMID: 22103483)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Prealbumin/transthyretin antibody 66108-1-Ig | Download protocol |
| IHC protocol for Prealbumin/transthyretin antibody 66108-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Am J Pathol Renal function in aged C57BL/6J mice is impaired by deposition of age-related apolipoprotein A-II amyloid independent of kidney aging |
