UBC13 Polyclonal antibody

UBC13 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 10243-1-AP

Print datasheet

Synonyms

BLU, UBC13, UbcH ben, UBE2N, Ubiquitin carrier protein N, Ubiquitin protein ligase N



经过测试的应用

Positive WB detected inmouse thymus tissue, human heart tissue, Jurkat cells, mouse small intestine tissue
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy UBE2N Ready-To-Use IHC Kit. UBE2N primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10243-1-AP targets UBC13 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB,ELISA
Cited Applications IHC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig
Immunogen UBC13 fusion protein Ag0292 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast)
Synonyms BLU, UBC13, UbcH ben, UBE2N, Ubiquitin carrier protein N, Ubiquitin protein ligase N
Calculated Molecular Weight 17 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC000396
Gene Symbol UBE2N
Gene ID (NCBI) 7334
RRIDAB_2288109
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP61088
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2N is a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair.

实验方案

Product Specific Protocols
WB protocol for UBC13 antibody 10243-1-APDownload protocol
IHC protocol for UBC13 antibody 10243-1-APDownload protocol
IP protocol for UBC13 antibody 10243-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Mol Immunol

The deubiquitinase OTUB1 augments NF-κB-dependent immune responses in dendritic cells in infection and inflammation by stabilizing UBC13.

Authors - Floriana Mulas
pigWB

Mol Cell Proteomics

The mechano-ubiquitinome of articular cartilage: Differential ubiquitination and activation of a group of ER-associated DUBs and ER stress regulators

Authors - Nitchakarn Kaokhum
mouseWB,IP

Nat Commun

FTO-dependent m6A modification of Plpp3 in circSCMH1-regulated vascular repair and functional recovery following stroke

Authors - Bin Li
humanWB,IHC

Mol Biomed

SP1 transcriptionally regulates UBE2N expression to promote lung adenocarcinoma progression

Authors - Jianjun Li