验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HepG2 cells |
| Positive IP detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55110-1-AP targets USP22 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP, CoIP |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ubiquitin specific peptidase 22 |
| Synonyms | Deubiquitinating enzyme 22, KIAA1063, Ubiquitin thiolesterase 22, USP22, USP3L |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 58-60 kDa |
| GenBank Accession Number | NM_015276 |
| Gene Symbol | USP22 |
| Gene ID (NCBI) | 23326 |
| RRID | AB_10859943 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UPT9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
USP22, also named as KIAA1063 and USP3L, belongs to the peptidase C19 family and UBP8 subfamily. It is histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. USP22 catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. It is recruited to specific gene promoters by activators such as MYC, where it is required for transcription. USP22 is required for nuclear receptor-mediated transactivation and cell cycle progression. USP22 has 2 isoforms with molecular mass of 58kDa and 60 kDa. The antibody is specific to USP22.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for USP22 antibody 55110-1-AP | Download protocol |
| IHC protocol for USP22 antibody 55110-1-AP | Download protocol |
| IP protocol for USP22 antibody 55110-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun ERAP1 promotes Hedgehog-dependent tumorigenesis by controlling USP47-mediated degradation of βTrCP. | ||
Int J Radiat Oncol Biol Phys USP11 exacerbates radiation induced pneumonitis by activating endothelial cell inflammatory response via OTUD5-STING signaling | ||
Food Funct Quercetin prevents the USP22-Snail1 signaling pathway to ameliorate diabetic tubulointerstitial fibrosis | ||
Cell Oncol (Dordr) The POU2F1/miR-4490/USP22 axis regulates cell proliferation and metastasis in gastric cancer. | ||
Eur J Pharmacol USP22 aggravated diabetic renal tubulointerstitial fibrosis progression through deubiquitinating and stabilizing Snail1
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