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- KD/KO Validated
VAV1 Polyclonal antibody
VAV1 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IP, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, K-562 cells, HL-60 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human breast cancer tissue, human brain tissue, human kidney tissue, human liver tissue, human heart tissue, human spleen tissue, human lung tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16364-1-AP targets VAV1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human |
| Cited Reactivity | human, rat, gecko |
| Immunogen | VAV1 fusion protein Ag9049 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | vav 1 guanine nucleotide exchange factor |
| Synonyms | Proto oncogene vav, VAV, VAV1 |
| Calculated Molecular Weight | 845 aa, 98 kDa |
| Observed Molecular Weight | 98 kDa |
| GenBank Accession Number | BC013361 |
| Gene Symbol | VAV1 |
| Gene ID (NCBI) | 7409 |
| RRID | AB_2213571 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P15498 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for VAV1 antibody 16364-1-AP | Download protocol |
| IHC protocol for VAV1 antibody 16364-1-AP | Download protocol |
| IP protocol for VAV1 antibody 16364-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Biol Chem Adult astrocytes from reptiles are resistant to proinflammatory activation via sustaining Vav1 expression.
| ||
Biochem Biophys Res Commun Vav1 expression is increased in esophageal squamous cell carcinoma and indicates poor prognosis. | ||
Oncotarget Multiple regulatory aspects of histone methyltransferase EZH2 in Pb-induced neurotoxicity. | ||
Clin Sci (Lond) Novel role of Vav1-Rac1 pathway in actin cytoskeleton regulation in Interleukin-13-induced minimal change-like nephropathy.
| ||
Cancer Cell Int STING pathway contributes to the prognosis of hepatocellular carcinoma and identification of prognostic gene signatures correlated to tumor microenvironment | ||
Neural Regen Res Vav1 promotes inflammation and neuronal apoptosis in cerebral ischemia/reperfusion injury by upregulating microglial and NLRP3 inflammasome activation |
