验证数据展示
经过测试的应用
| Positive WB detected in | rat plasma, human plasma, human testis tissue, NIH/3T3 cells |
| Positive IHC detected in | human liver tissue, human hepatocirrhosis tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NIH/3T3 cells |
Planning an IHC experiment? We recommend our IHCeasy VTN Ready-To-Use IHC Kit. VTN primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15833-1-AP targets Vitronectin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, rat, pig, plasmodium falciparum |
| 免疫原 | Vitronectin fusion protein Ag8443 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | vitronectin |
| 别名 | Vitronectin V65 subunit, Vitronectin V10 subunit, V75, S-protein, Somatomedin-B |
| 计算分子量 | 478 aa, 54 kDa |
| 观测分子量 | 70-75 kDa, 65 kDa, |
| GenBank蛋白编号 | BC005046 |
| 基因名称 | VTN |
| Gene ID (NCBI) | 7448 |
| RRID | AB_2216309 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P04004 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Vitronectin is a 75-kDa glycoprotein present in blood and in the extracellular matrix (PMID: 10399314). Vitronectin is a multifunctional glycoprotein that mediates cell-to-substrate adhesion, inhibits the cytolytic action of the terminal complement cascade in vitro and binds to several serine protease inhibitors of the serpin family (PMID: 1372588).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Vitronectin antibody 15833-1-AP | Download protocol |
| IHC protocol for Vitronectin antibody 15833-1-AP | Download protocol |
| IF protocol for Vitronectin antibody 15833-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Stem Cell Region-specific cellular and molecular basis of liver regeneration after acute pericentral injury | ||
Brain Pathol Reconstituting neurovascular unit with primary neural stem cells and brain microvascular endothelial cells in three-dimensional matrix. | ||
Sci Rep Molecular Camouflage of Plasmodium falciparum Merozoites by Binding of Host Vitronectin to P47 Fragment of SERA5. | ||
J Cell Mol Med Induction of migration of periodontal ligament cells by selective regulation of integrin subunits. | ||
Langmuir Characterization of Initial Cell Adhesion on Charged Polymer Substrates in Serum-Containing and Serum-Free Media. | ||
Proteomics SILAC-based Quantitative Proteomic Analysis of Secretome between Activated and Reverted Hepatic Stellate Cells. |
