验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH3T3 cells, RAW 264.7 cells |
Positive IHC detected in | human lung cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | MCF-7 cells |
Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67698-1-Ig targets YWHAZ in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Tested Reactivity | Human, mouse, rat |
Immunogen | YWHAZ fusion protein Ag6667 种属同源性预测 |
Host / Isotype | Mouse / IgM |
Class | Monoclonal |
Type | Antibody |
Full Name | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide |
Synonyms | 14 3 3 protein zeta/delta, KCIP 1, YWHAZ |
Calculated Molecular Weight | 28 kDa |
Observed Molecular Weight | 28-30 kDa |
GenBank Accession Number | BC003623 |
Gene Symbol | YWHAZ |
Gene ID (NCBI) | 7534 |
RRID | AB_2882890 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Caprylic acid/ammonium sulfate precipitation |
UNIPROT ID | P63104 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
YWHAZ (also known as 14-3-3 zeta) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis.
实验方案
Product Specific Protocols | |
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WB protocol for YWHAZ antibody 67698-1-Ig | Download protocol |
IHC protocol for YWHAZ antibody 67698-1-Ig | Download protocol |
IF protocol for YWHAZ antibody 67698-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |