- Featured Product
- KD/KO Validated
ZO-1 Monoclonal antibody
ZO-1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, canine and More (1)
Applications
WB, IHC, IF/ICC, IF-P, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
1G4A1
验证数据展示
经过测试的应用
Positive WB detected in | HUVEC cells, HeLa cells, COLO 320 cells, A431 cells, LNCaP cells, HEK-293 cells, HepG2 cells |
Positive IHC detected in | human pancreas cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human breast cancer tissue |
Positive IF/ICC detected in | MCF-7 cells, MDCK cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66452-1-Ig targets ZO-1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, canine samples.
Tested Applications | WB, IHC, IF/ICC, IF-P, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, canine |
Cited Reactivity | human, pig |
Immunogen | ZO-1 fusion protein Ag16454 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | tight junction protein 1 (zona occludens 1) |
Synonyms | TJP1, ZO 1, Tight junction protein ZO-1, Tight junction protein ZO 1, Tight junction protein 1 |
Calculated Molecular Weight | 1748 aa, 195 kDa |
Observed Molecular Weight | 230 kDa |
GenBank Accession Number | BC111712 |
Gene Symbol | ZO-1 |
Gene ID (NCBI) | 7082 |
RRID | AB_2881821 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q07157 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for ZO-1 antibody 66452-1-Ig | Download protocol |
IHC protocol for ZO-1 antibody 66452-1-Ig | Download protocol |
IF protocol for ZO-1 antibody 66452-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Sci Adv Astrocytic NDRG2-PPM1A interaction exacerbates blood-brain barrier disruption after subarachnoid hemorrhage | ||
Nat Commun An electrochemical biosensor for the detection of epithelial-mesenchymal transition. | ||
Cell Death Differ SARS-CoV-2 spike protein dictates syncytium-mediated lymphocyte elimination. | ||
ACS Appl Mater Interfaces Betaine-Based and Polyguanidine-Inserted Zwitterionic Micelle as a Promising Platform to Conquer the Intestinal Mucosal Barrier | ||
Int J Biol Macromol zwitterionic Pluronic analog-coated PLGA nanoparticles for oral insulin delivery | ||
Cell Mol Life Sci Silencing circSERPINE2 restrains mesenchymal stem cell senescence via the YBX3/PCNA/p21 axis |