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NeutraKine® Beta NGF Monoclonal antibody

NeutraKine® Beta NGF Monoclonal Antibody for Neutralization, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

Neutralization, ELISA

Conjugate

Unconjugated

CloneNo.

4F9H1

Cat No : 69009-1-Ig

Print datasheet

Synonyms

Beta nerve growth factor, Beta NGF, HSAN5, NeutraKine® Beta NGF, NGF, NGFB, proNGF

验证数据展示

  • Neutralization experiment of NeutraKine® Beta NGF using 69009-1-Ig

    Neutralization experiment of NeutraKine® Beta NGF using 69009-1-Ig

    Recombinant human beta-NGF (Cat.NO. HZ-1222) stimulates proliferation of TF-1 cell line (human erythroleukemic cell line) in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human beta-NGF (2 ng/mL HZ-1222) is neutralized by mouse anti-human beta-NGF monoclonal antibody 69009-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 4-20 ng/mL.

NeutralizationMeasured by its ability to neutralize Human beta-NGF induced proliferation of TF-1 cell line (human erythroleukemic cell line) . The half neutralization dose (ND50) is typically 4-20 ng/mL in the presence of 2 ng/mL human beta-NGF.

产品信息

69009-1-Ig targets NeutraKine® Beta NGF in Neutralization, ELISA applications and shows reactivity with Human samples.

Tested Applications Neutralization, ELISA
Tested Reactivity Human
Immunogen human Humankine Beta NGF protein HZ-1222 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name nerve growth factor (beta polypeptide)
Synonyms Beta nerve growth factor, Beta NGF, HSAN5, NeutraKine® Beta NGF, NGF, NGFB, proNGF
Gene Symbol NGF
Gene ID (NCBI) 4803
RRIDAB_2882927
Conjugate Unconjugated
Form Lyophilized Powder
Purification MethodProtein G purification
Storage Buffer Sterile PBS.
Endotoxin<0.1 EU/μg
ReconstitutionThis product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
Stability and StorageLyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

背景介绍

Beta nerve growth factor (NGF) is critical for the survival and maintenance of sympathetic and sensory neurons and may play an important role in the regulation of the immune system (PMID 16842161). The presence of beta NGF in immune cells, endocrine cells, and the CNS limbic areas suggests that beta NGF may function as an intracellular messenger to regulate the body's response to stress (PMID 19442684).

This antibody can be used to neutralize the bioactivity of Beta NGF.