c-SRC Monoclonal antibody

c-SRC Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, hamster, mouse, rat

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

5E10C4

Cat No : 60315-1-Ig

Print datasheet

Synonyms

ASV, c SRC, c-SRC, p60 Src, pp60c src, Proto oncogene c Src, SRC, SRC1



经过测试的应用

Positive WB detected inHeLa cells, RAW 264.7 cells, CHO cells, HepG2 cells, HEK-293 cells, Jurkat cells, NIH/3T3 cells, 4T1 cells, PC-12 cells, HSC-T6 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy c-SRC Ready-To-Use IHC Kit. c-SRC primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60315-1-Ig targets c-SRC in WB, IHC, IF, ELISA applications and shows reactivity with human, hamster, mouse, rat samples.

Tested Applications IF, IHC, WB, ELISA
Cited Applications IF, WB
Tested Reactivity human, hamster, mouse, rat
Cited Reactivityhuman, mouse
Immunogen c-SRC fusion protein Ag22306 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
Synonyms ASV, c SRC, c-SRC, p60 Src, pp60c src, Proto oncogene c Src, SRC, SRC1
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC011566
Gene Symbol SRC
Gene ID (NCBI) 6714
ENSEMBL Gene IDENSG00000197122
RRIDAB_2881426
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP12931
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase.

实验方案

Product Specific Protocols
WB protocol for c-SRC antibody 60315-1-IgDownload protocol
IHC protocol for c-SRC antibody 60315-1-IgDownload protocol
IF protocol for c-SRC antibody 60315-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Hepatology

Basolateral CD147 induces hepatocyte polarity loss by E-cadherin ubiquitination and degradation in hepatocellular carcinoma progress.

Authors - Meng Lu
WB

Cancer Res

Osimertinib covalently binds to CD34 and eliminates myeloid leukemia stem/progenitor cells

Authors - Li Xia
humanWB

Nat Commun

Phospho-selective mechanisms of arrestin conformations and functions revealed by unnatural amino acid incorporation and (19)F-NMR.

Authors - Fan Yang
mouseWB

EMBO Mol Med

Inhibition of Sema4D/PlexinB1 signaling alleviates vascular dysfunction in diabetic retinopathy.

Authors - Jie-Hong Wu
humanWB

Oncogene

KIF26B, a novel oncogene, promotes proliferation and metastasis by activating the VEGF pathway in gastric cancer.

Authors - H Zhang
humanWB

Cancers (Basel)

Adherens Junction Integrity Is a Critical Determinant of Sodium Iodide Symporter Residency at the Plasma Membrane of Thyroid Cells

Authors - Márcia Faria