aequorea victoria GFP tag Polyclonal antibody
GFP tag Polyclonal Antibody for IF/ICC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
aequorea victoria, recombinant protein and More (5)
Applications
IF/ICC, IP, WB, ELISA and More (5)
Conjugate
Unconjugated
580
验证数据展示
经过测试的应用
Positive WB detected in | recombinant protein, Transfected HEK-293 cells |
Positive IP detected in | Transfected HEK-293 cells |
Positive IF detected in | Transfected HEK-293 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF) | IF : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
50430-2-AP targets GFP tag in WB,IP,IF,RIP,IHC,CoIP,ChIP,IP-MS,ELISA applications and shows reactivity with aequorea victoria, recombinant protein samples.
Tested Applications | IF/ICC, IP, WB, ELISA |
Cited Applications | WB,IP,IF,RIP,IHC,CoIP,ChIP,IP-MS |
Tested Reactivity | aequorea victoria, recombinant protein |
Cited Reactivity | mouse, rat, yeast, monkey, hamster |
Immunogen | GFP tag fusion protein Ag2128 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | GFP tag |
Synonyms | CFP, eGFP, eYFP, GFP, GFP tag, YFP |
Calculated Molecular Weight | 26 kDa |
GenBank Accession Number | U73901 |
Gene Symbol | |
Gene ID (NCBI) | |
RRID | AB_11042881 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em). This antibody is a rabbit polyclonal antibody raised against full-length eGFP and reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP and eGFP.
实验方案
Product Specific Protocols | |
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WB protocol for GFP tag antibody 50430-2-AP | Download protocol |
IF protocol for GFP tag antibody 50430-2-AP | Download protocol |
IP protocol for GFP tag antibody 50430-2-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Gastroenterology PTEN deficiency facilitates exosome secretion and metastasis in cholangiocarcinoma by impairing TFEB-mediated lysosome biogenesis | ||
Nat Genet Pathogenic SPTBN1 variants cause an autosomal dominant neurodevelopmental syndrome. | ||
Cell Stem Cell DUX-miR-344-ZMYM2-Mediated Activation of MERVL LTRs Induces a Totipotent 2C-like State. | ||
Nat Methods TALEN-mediated precise genome modification by homologous recombination in zebrafish. |