GFP tag Polyclonal antibody

GFP tag Polyclonal Antibody for WB, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

aequorea victoria, recombinant protein and More (6)

Applications

WB, IF/ICC, IF-P, IP, ELISA and More (6)

Conjugate

Unconjugated

Cat no : 50430-2-AP

Print datasheet

Synonyms

eGFP, GFP, green, GFP-tag, eYFP



经过测试的应用

Positive WB detected inrecombinant protein, Transfected HEK-293 cells
Positive IP detected inTransfected HEK-293T cells
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inTransfected HEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

50430-2-AP targets GFP tag in WB, IHC, IF/ICC, IF-P, IP, CoIP, ChIP, RIP, IP-MS, ELISA applications and shows reactivity with aequorea victoria, recombinant protein samples.

Tested Applications WB, IF/ICC, IF-P, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP, ChIP, RIP, IP-MS
Tested Reactivity aequorea victoria, recombinant protein
Cited Reactivitymouse, rat, pig, monkey, hamster, yeast
Immunogen GFP tag fusion protein Ag2128 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GFP tag
Synonyms eGFP, GFP, green, GFP-tag, eYFP
Calculated Molecular Weight 26 kDa
GenBank Accession NumberM62653
Gene Symbol
Gene ID (NCBI)
RRIDAB_11042881
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages. 

Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm.

When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.

This antibody is a rabbit polyclonal antibody, generated against the full-length eGFP protein. It exhibits reactivity towards variants of Aequorea victoria GFP, including S65T-GFP, RS-GFP, YFP, CFP, and eGFP.


实验方案

Product Specific Protocols
WB protocol for GFP tag antibody 50430-2-APDownload protocol
IF protocol for GFP tag antibody 50430-2-APDownload protocol
IP protocol for GFP tag antibody 50430-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,hamsterWB

Nat Biotechnol

Programmable protein expression using a genetically encoded m6A sensor

Authors - Bahjat F Marayati
WB

Cell

Phase separation drives RNA virus-induced activation of the NLRP6 inflammasome.

Authors - Chen Shen
WB,IF

Gastroenterology

PTEN deficiency facilitates exosome secretion and metastasis in cholangiocarcinoma by impairing TFEB-mediated lysosome biogenesis

Authors - Tian-Yi Jiang
WB

Nat Genet

Pathogenic SPTBN1 variants cause an autosomal dominant neurodevelopmental syndrome.

Authors - Margot A Cousin
IF

Cell Stem Cell

DUX-miR-344-ZMYM2-Mediated Activation of MERVL LTRs Induces a Totipotent 2C-like State.

Authors -
WB

Nat Methods

TALEN-mediated precise genome modification by homologous recombination in zebrafish.

Authors - Zu Yao Y