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Lamin A/C Polyclonal antibody

Lamin A/C Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

FC, IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 10298-1-AP

Print datasheet

Synonyms

70 kDa lamin, CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, HGPS, IDC, lamin A, lamin A/C, LDP1, LFP, LGMD1B, LMN1, LMNA, LMNC, Prelamin A/C, PRO1, progerin



经过测试的应用

Positive WB detected inA431 cells, HEK-293 cells, HUVEC cells, mouse ovary tissue, SKOV-3 cells, HeLa cells, A375 cells, C6 cells, NIH/3T3 cells
Positive IP detected inHeLa cells, A375 cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HeLa cells
Positive FC detected inHEK-293T cells
Planning an IHC experiment? We recommend our IHCeasy Lamin A/C Ready-To-Use IHC Kit. Lamin A/C primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:400-1:1600
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10298-1-AP targets Lamin A/C in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited Applications IF, IHC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey, duck
Immunogen Lamin A/C fusion protein Ag0408 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lamin A/C
Synonyms 70 kDa lamin, CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, HGPS, IDC, lamin A, lamin A/C, LDP1, LFP, LGMD1B, LMN1, LMNA, LMNC, Prelamin A/C, PRO1, progerin
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 65 kDa, 70 kDa
GenBank Accession NumberBC003162
Gene Symbol LMNA
Gene ID (NCBI) 4000
ENSEMBL Gene IDENSG00000160789
RRIDAB_2296961
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP02545
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lamin A/C is also named as LMNA or LMN1. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure, and gene expression. The lack of lamin A/C can be as a novel marker for undifferentiated embryonic stem cells and lamin A/C expression is an early indicator of differentiation (PMID: 16179429). Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb-girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. This protein has 4 isoforms produced by alternative splicing with the molecular weight of 74 kDa, 65 kDa, 70 kDa, and 64 kDa. This antibody can recognize 4 isoforms of Lamin A/C.

实验方案

Product Specific Protocols
WB protocol for Lamin A/C antibody 10298-1-APDownload protocol
IHC protocol for Lamin A/C antibody 10298-1-APDownload protocol
IF protocol for Lamin A/C antibody 10298-1-APDownload protocol
IP protocol for Lamin A/C antibody 10298-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nat Microbiol

Nuclear pore blockade reveals that HIV-1 completes reverse transcription and uncoating in the nucleus.

Authors - Adarsh Dharan
humanWB

Mol Cell

Lactylation-driven METTL3-mediated RNA m6A modification promotes immunosuppression of tumor-infiltrating myeloid cells.

Authors - Jia Xiong
humanWB

J Extracell Vesicles

Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway.

Authors - Xiaohan Gao
humanWB

Cancer Cell

SET1A-Mediated Mono-Methylation at K342 Regulates YAP Activation by Blocking Its Nuclear Export and Promotes Tumorigenesis.

Authors - Lan Fang
humanWB

Sci Adv

XAF1 promotes anti-RNA virus immune responses by regulating chromatin accessibility

Authors - Ming Kuang
humanWB

Autophagy

SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes

Authors - Wang Zheng