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验证数据展示

  • IF Staining of A431 using CL488-12143

    IF Staining of A431 using CL488-12143

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 p63 antibody (CL488-12143) at dilution of 1:1000, CL594-Phalloidin (red).

  • FC experiment of A431 using CL488-12143

    FC experiment of A431 using CL488-12143

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human p63 (CL488-12143) (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated p63 Polyclonal antibody

p63 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-12143

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

AIS, B(p51A), B(p51B), CUSP, deltaNp63, EEC3, KET, LMS, NBP, OFC8, p40, p51, p53CP, p63, p73H, p73L, RHS, SHFM4, TP53CP, TP53L, TP63, TP73L, Tumor protein 63, tumor protein p63, Tumor protein p73 like

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells
Positive FC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy TP63 Ready-To-Use IHC Kit. TP63 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 12143-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-12143 targets p63 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen p63 fusion protein Ag2791 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tumor protein p63
Synonyms AIS, B(p51A), B(p51B), CUSP, deltaNp63, EEC3, KET, LMS, NBP, OFC8, p40, p51, p53CP, p63, p73H, p73L, RHS, SHFM4, TP53CP, TP53L, TP63, TP73L, Tumor protein 63, tumor protein p63, Tumor protein p73 like
Calculated Molecular Weight 680 aa, 77 kDa
GenBank Accession NumberBC039815
Gene Symbol TP63
Gene ID (NCBI) 8626
RRIDAB_2883081
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H3D4
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TP63, also named KET, P63, P73H, P73L and TP73L, belongs to the p53 family. It is a homologue of the tumor suppressor p53 and p73 genes. It is involved in malignancy acquisition and maintenance of cells. Unlike p53, the p63 gene encodes multiple isotypes with remarkably divergent abilities to transactivate p53 reporter genes and induce apoptosis. TP63 acts as a sequence specific DNA binding transcriptional activator or repressor. This antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 p63 antibody CL488-12143Download protocol
FC protocol for CL Plus 488 p63 antibody CL488-12143Download protocol
Standard Protocols
Click here to view our Standard Protocols