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ERp19 Polyclonal antibody

ERp19 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 20181-1-AP

Print datasheet

Synonyms

AGR1, AIF, AIFM1, ERp18, ERp19, hAG 1, PDCD8, PDIA16, TLP19, TXNDC12

验证数据展示

  • WB analysis of human brain using 20181-1-AP

    WB analysis of human brain using 20181-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20181-1-AP (ERp19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 20181-1-AP

    IHC staining of human liver cancer using 20181-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20181-1-AP (ERp19 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TXNDC12 antibody included
  • IHC staining of human liver cancer using 20181-1-AP

    IHC staining of human liver cancer using 20181-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20181-1-AP (ERp19 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TXNDC12 antibody included

经过测试的应用

Positive WB detected inhuman brain tissue, human heart tissue, mouse kidney tissue, mouse lung tissue
Positive IHC detected inhuman liver cancer tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TXNDC12 Ready-To-Use IHC Kit. TXNDC12 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20181-1-AP targets ERp19 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen ERp19 fusion protein Ag14045 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name thioredoxin domain containing 12 (endoplasmic reticulum)
Synonyms AGR1, AIF, AIFM1, ERp18, ERp19, hAG 1, PDCD8, PDIA16, TLP19, TXNDC12
Calculated Molecular Weight 172 aa, 19 kDa
Observed Molecular Weight 20 kDa, 40 kDa
GenBank Accession NumberBC001493
Gene Symbol TXNDC12
Gene ID (NCBI) 51060
RRIDAB_10646468
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95881
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TXNDC12, which is known as thioredoxin domain-containing protein 12, ERp16, ERp18, ERp19, or hTLP19, is a member of the protein disulfide isomerase (PDI) family that plays important roles in cancer development and progression. TXNDC12 has been demonstrated to contribute to tumorigenicity in human gastric cancer by promoting cell growth, migration, and invasion(PMID: 25940440). Other studies also revealed the overexpression of TXNDC12 promotes HCC metastasis through β-catenin-mediated induction of EMT and that TXNDC12 has the potential to be a prognostic factor and therapeutic target of HCC. (PMID: 31570854)

实验方案

Product Specific Protocols
WB protocol for ERp19 antibody 20181-1-APDownload protocol
IHC protocol for ERp19 antibody 20181-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols