Various lysates were subjected to SDS PAGE followed by western blot with 11265-1-AP (CD31 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11265-1-AP (CD31 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11265-1-AP (CD31 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IP result of anti-CD31 (IP:11265-1-AP, 5ug; Detection:11265-1-AP 1:300) with Jurkat cells lysate 4000ug.

Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using CD31 antibody (11265-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

1X10^6 HUVEC cells were stained with 0.2ug CD31 antibody (11265-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

bEnd.3 cells were subjected to SDS PAGE followed by western blot with 28083-1-AP (CD31 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28083-1-AP (CD31 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28083-1-AP (CD31 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CD31 antibody (28083-1-AP) at dilution of 1:8000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CD31 antibody (28083-1-AP) at dilution of 1:8000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using KHC0022 (CD31 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using KHC0022 (CD31 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using KHC0022 (CD31 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using KHC0022 (CD31 IHC Kit).

Untreated and PNGase F-treated lysates of human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66065-2-Ig (CD31 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66065-2-Ig (CD31 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66065-2-Ig (CD31 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CD31 antibody (66065-2-Ig, Clone: 3F8E2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CD31 antibody (66065-2-Ig, Clone: 3F8E2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

1X10^6 THP-1 cells were surface stained with 0.4 ug Anti-Human CD31 (66065-2-Ig, Clone:3F8E2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were not fixed.

Untreated and PNGase F-treated lysates of Jurkat cells were subjected to SDS PAGE followed by western blot with 66065-2-Ig (CD31 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Lymphatic endothelial cells were stained for CD31 (green, Cat. No CL488-66065, 1:200) and alpha tubulin (magenta, Cat. 11224-1-AP, 1:200) with DAPI as a counterstain for visualizing the nucleus (yellow). CD31 is an endothelial cell adhesion protein and was conjugated to CoraLite-488 fluorescent dye to allow for direct visualization. Alpha tubulin is a cytoskeletal protein important in cell division and migration and was visualized with an Alexa Fluor 568 secondary antibody. Cells were fixed with 4% paraformaldehyde. Image credit: @Immunofluorescence on Instagram

Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488 CD31 antibody (CL488-66065, Clone: 2A1E2 ) at dilution of 1:400.

1X10^6 THP-1 cells were surface stained with 0.4 ug CoraLite® Plus 488 Anti-Human CD31 (CL488-66065, Clone:3F8E2) (red), or 0.4 ug Control Antibody. Cells were not fixed.

Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD31 antibody (CL594-66065, Clone: 2A1E2 ) at dilution of 1:200.

Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD31 antibody (CL594-66065, Clone: 2A1E2 ) at dilution of 1:200.

G66065-2-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

G66065-2-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

G66065-2-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

G66065-2-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

1X10^6 C57BL/6 mouse bone marrow cells were surface co-stained with APC Anti-Mouse CD45R (B220) and 0.5 ug FITC Plus Anti-Mouse CD31 (FITC-65058, Clone: 390) or 0.5 ug FITC Rat IgG2a Isotype Control (2A3) (FITC-65209, Clone: 2A3). Cells were not fixed.

Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using FITC Anti-Mouse CD31 (FITC-65058, Clone: 390) at dilution of 1:100.