Sam68 Polyclonal antibody
Sam68 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC, IF/ICC, IHC, IP, WB, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HT-29 cells, mouse brain tissue, HeLa cells, NIH/3T3 cells, HEK-293 cells, rat brain tissue |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | mouse brain tissue |
Positive FC detected in | MCF-7 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10222-1-AP targets Sam68 in WB, IP, IF, FC, RIP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC, IF/ICC, IHC, IP, WB, ELISA |
Cited Applications | WB, RIP, IHC, CoIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | Sam68 fusion protein Ag0235 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | KH domain containing, RNA binding, signal transduction associated 1 |
Synonyms | KHDRBS1, p62, p68, Sam68 |
Calculated Molecular Weight | 48 kDa |
Observed Molecular Weight | 68 kDa |
GenBank Accession Number | BC000717 |
Gene Symbol | KHDRBS1 |
Gene ID (NCBI) | 10657 |
RRID | AB_2130292 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q07666 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Sam68 antibody 10222-1-AP | Download protocol |
IHC protocol for Sam68 antibody 10222-1-AP | Download protocol |
IF protocol for Sam68 antibody 10222-1-AP | Download protocol |
IP protocol for Sam68 antibody 10222-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Acta Neuropathol Transportin 1 accumulates specifically with FET proteins but no other transportin cargos in FTLD-FUS and is absent in FUS inclusions in ALS with FUS mutations. | ||
Theranostics SAM68 promotes tumorigenesis in lung adenocarcinoma by regulating metabolic conversion via PKM alternative splicing. | ||
Mol Ther Nucleic Acids LRRC4 mediates the formation of circular RNA CD44 to inhibitGBM cell proliferation | ||
Reprod Fertil Dev Cold-induced RNA-binding protein (CIRBP) regulates the expression of Src-associated during mitosis of 68 kDa (Sam68) and extracellular signal-regulated kinases (ERK) during heat stress-induced testicular injury. |