LC3A-Specific Polyclonal antibody
LC3A-Specific Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
FC, IF, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, human brain tissue, rat brain tissue |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 9 publications below |
IHC | See 1 publications below |
产品信息
18722-1-AP targets LC3A-Specific in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC, IF, IHC, WB, ELISA |
Cited Applications | WB, IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, zebrafish |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | microtubule-associated protein 1 light chain 3 alpha |
Synonyms | LC3, LC3A, LC3A-Specific, MAP1A/MAP1B LC3 A, MAP1A/MAP1B light chain 3 A, MAP1ALC3, MAP1BLC3, MAP1LC3A |
Calculated Molecular Weight | 14 kDa |
Observed Molecular Weight | 15-18 kDa |
GenBank Accession Number | NM_032514 |
Gene Symbol | MAP1LC3A |
Gene ID (NCBI) | 84557 |
RRID | AB_10597104 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9H492 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for LC3A-Specific antibody 18722-1-AP | Download protocol |
IHC protocol for LC3A-Specific antibody 18722-1-AP | Download protocol |
IF protocol for LC3A-Specific antibody 18722-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Oxid Med Cell Longev Autophagy Triggered by Oxidative Stress Appears to Be Mediated by the AKT/mTOR Signaling Pathway in the Liver of Sleep-Deprived Rats. | ||
Oxid Med Cell Longev Imbalance of Autophagy and Apoptosis Induced by Oxidative Stress May Be Involved in Thyroid Damage Caused by Sleep Deprivation in Rats. | ||
Front Cell Dev Biol Tcap Deficiency in Zebrafish Leads to ROS Production and Mitophagy, and Idebenone Improves its Phenotypes. | ||
J Cell Mol Med Connexin 31.1 degradation requires the Clathrin-mediated autophagy in NSCLC cell H1299. | ||
Mar Drugs 7-Acetylsinumaximol B Induces Apoptosis and Autophagy in Human Gastric Carcinoma Cells through Mitochondria Dysfunction and Activation of the PERK/eIF2α/ATF4/CHOP Signaling Pathway. | ||
Mol Cell Neurosci Elevated ATG13 in serum of patients with ME/CFS stimulates oxidative stress response in microglial cells via activation of receptor for advanced glycation end products (RAGE). |