human YWHAE-6*His fusion protein
E. coli-derived, PET28a
(1-255 aa encoded by BC000179
Please contact the lab for more information.
95%, by SDS-PAGE with Coomassie Brilliant Blue staining.
Protein lyophilized in sterile PBS (58 mM Na2HPO4, 17 mM NaH2PO4, 68 mM NaCl, 300 mM Imidazole, pH 8.0). Trehalose (5-8%) and mannitol (5-8%) protectants were added before lyophilization.
Reconstitution and Storage
Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
Reconstitution with 200 μl 50% glycerol solution is recommended for longer term
storage (see Stability and Storage for more details).
If a different concentration is needed for your purposes please adjust the reconstitution
volume as required (please note: the ion concentration of the final solution will
vary according to the volume used).
Note: Centrifuge vial before opening. When reconstituting, gently pipet and wash
down the sides of the vial to ensure full recovery of the protein into solution.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature (see below).
Stability and Storage:
Store for up to 12 months at -20°C to -80°C as lyophilized powder.
Storage of Reconstituted Protein:
Short-term storage: Store at 2-8°C for (1-2 weeks).
Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer
containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw