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4EBP1 Monoclonal antibody

4EBP1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat, pig, mouse and More (1)

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2C3F3

Cat No : 60246-1-Ig

Print datasheet

Synonyms

4E BP1, 4EBP1, BP 1, eIF4E binding protein 1, EIF4EBP1, PHAS I



经过测试的应用

Positive WB detected inK-562 cells, RAW 264.7 cells, NCI-H1299 cells, HCT 116 cells, C6 cells, K562
Positive IHC detected inhuman pancreas cancer tissue, mouse liver tissue, rat liver tissue, rat pancreas tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60246-1-Ig targets 4EBP1 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat, pig, mouse samples.

Tested Applications IF, IHC, WB, ELISA
Cited Applications IHC, WB
Tested Reactivity human, rat, pig, mouse
Cited Reactivityhuman, chicken
Immunogen 4EBP1 fusion protein Ag18985 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name eukaryotic translation initiation factor 4E binding protein 1
Synonyms 4E BP1, 4EBP1, BP 1, eIF4E binding protein 1, EIF4EBP1, PHAS I
Calculated Molecular Weight 118 aa, 12 kDa
Observed Molecular Weight 15-24 kDa
GenBank Accession NumberBC004459
Gene Symbol EIF4EBP1
Gene ID (NCBI) 1978
RRIDAB_2881368
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13541
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Eukaryotic translation initiation factor 4E-binding protein 1(4EBP1), is a member of 4EBPs family, which regulate the translation of a subset of mRNA by competing with eIF4G for binding to eIF4E, thus preventing the assembly of the eIF4F complex. The eIF4F facilitates the recruitment of other translation initiation factors to form the complex and then initiates cap-dependent translation.4EBP1 also mediates the regulation of protein translation by growth factors, hormones and other stimuli that signal through the MAP kinase and mTORC1 pathways. There are three forms of 4EBP1, alpha, beta and gamma.typical pattern seen on Western blots for phosphorylated heat-acid stabled protein 4EBP1 from rat gastrocnemius muscle. Three distinct bands are noted, with the most rapidly migrating band having an apparent molecular mass of ; 20 kDa and the slowest migrating band of; 24 kDa. The 3 bands are designated by convention asalpha, beta and gamma. (PMID: 10913029)

实验方案

Product Specific Protocols
WB protocol for 4EBP1 antibody 60246-1-IgDownload protocol
IHC protocol for 4EBP1 antibody 60246-1-IgDownload protocol
IF protocol for 4EBP1 antibody 60246-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Nature

Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis.

Authors - Xiao-Yi Lu

J Hepatol

OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.

Authors - Weiqi Dai
humanIHC

Mol Cancer

The circular RNA circ-ERBIN promotes growth and metastasis of colorectal cancer by miR-125a-5p and miR-138-5p/4EBP-1 mediated cap-independent HIF-1α translation.

Authors - Liang-Yan Chen
humanWB

Cell Death Differ

Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration

Authors - Huaizhen Liang
humanWB

Autophagy

STYK1 promotes autophagy through enhancing the assembly of autophagy-specific class III phosphatidylinositol 3-kinase complex I.

Authors - Cefan Zhou
WB,IHC

Toxicology

Toxicity mechanism of peri-implantation pesticide beta-cypermethrin exposure on endometrial remodeling in early pregnant mice

Authors - Yong-Jiang Zhou