5-mCpA antibody (mAb) (Clone 2C8H8A6)
Host / Isotype
Mouse / IgG1
Reactivity
Not Species Specific
Applications
DB
CloneNo.
2C8H8A6
Cat No : 61783,61784 61783
Synonyms
验证数据展示
产品信息
| Tested Applications |
DB
Applications Validated by Active Motif: DB: 1 ug/ml |
| Tested Reactivity | Not Species Specific |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Modification | Methylated |
| Immunogen | This antibody was raised against 5-methyl-cytosine-adenosine di-nucleoside conjugated to KLH and recognized 5-mCpA. |
| Full Name | 5-mCpA antibody (mAb) (Clone 2C8H8A6) |
| Synonyms | CpA Dinucleotide, Dinucleotide island, CpG, CpA |
| Molecular weight | |
| GenBank accession number | N/A | RRID | AB_2793764 | Purification Method | Protein A Chromatography |
| Buffer | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
背景介绍
DNA methylation is important for regulation of transcription, and in processes including imprinting, gene silencing and cancer development. Methylation occurs predominantly at cytosine within the dinucleotide CpG (meCpG), which is frequently found in promoter regions near transcription start sites, as well as in promoters for functional non-coding RNAs. However, methylation at CpG dinucleotides makes them susceptible to both spontaneous deamination and enzyme-mediated deamination, resulting in thymine substitution (T/G mismatch) and the formation of a CpA dinucleotide in the opposite strand. Therefore, there is a strong correlation of CpG dinucleotide depletion or “suppression” with an observed increase in TpG/CpA dinucleotides.In mammals, there are certain cell types in which significant levels of methylation at CpA, (meCpA), CpT (meCpT), CpC (meCpC) is also observed, including embryonic stem cells, oocytes, primordial germ cells and neurons. Early observations suggest that meCpA, in particular, has different nuclear distribution than meCpG, and that meCpA may associate with active transcription rather than suppression. DNA methylation is important for regulation of transcription, and in processes including imprinting, gene silencing and cancer development. Methylation occurs predominantly at cytosine within the dinucleotide CpG (meCpG), which is frequently found in promoter regions near transcription start sites, as well as in promoters for functional non-coding RNAs. However, methylation at CpG dinucleotides makes them susceptible to both spontaneous deamination and enzyme-mediated deamination, resulting in thymine substitution (T/G mismatch) and the formation of a CpA dinucleotide in the opposite strand. Therefore, there is a strong correlation of CpG dinucleotide depletion or “suppression” with an observed increase in TpG/CpA dinucleotides.In mammals, there are certain cell types in which significant levels of methylation at CpA, (meCpA), CpT (meCpT), CpC (meCpC) is also observed, including embryonic stem cells, oocytes, primordial germ cells and neurons. Early observations suggest that meCpA, in particular, has different nuclear distribution than meCpG, and that meCpA may associate with active transcription rather than suppression.