Name: ACADM Mouse Monoclonal antibody
Brand: Proteintech

Applications

Tested Applications:

IHC, WB, ELISA
Application not tested? Please see our Guarantee

Various lysates were subjected to SDS PAGE followed by western blot with 67742-1-Ig (ACADM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

WB analysis of rat liver using 67742-1-Ig

rat liver tissue were subjected to SDS PAGE followed by western blot with 67742-1-Ig (ACADM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

IHC staining of human liver using 67742-1-Ig

Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67742-1-Ig (ACADM antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67742-1-Ig (ACADM antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67742-1-Ig (ACADM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Species specificity:

Human

Positive WB detected in:

PC-3 cells, HSC-T6 cells, rat liver tissue

Positive IHC detected in:

human liver tissue, human heart tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution:

WB : 1:2000-1:10000

IHC : 1:250-1:1000

Product Information

Source:

Mouse

Purification method:

Protein G purification

Isotype:

IgG1

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

ACADM fusion protein Ag30620

Full name:

acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain

Calculated molecular weight:

421 aa, 47 kDa

Observed molecular weight:

42 kDa

GenBank accession number:

BC005377

Gene ID (NCBI):

34

Gene symbol

ACADM

Synonyms

ACAD1, ACADM, FLJ18227, FLJ93013, FLJ99884, MCAD, MCADH

Background

ACADM, also named as MCAD, belongs to the acyl-CoA dehydrogenase family. This enzyme is specific for acyl chain lengths of 4 to 16. It catalyzes the reaction: Acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor. Defects in ACADM are the cause of medium-chain acyl-CoA dehydrogenase deficiency (MCAD deficiency). This protein can exsit as a dimer(PMID:8962055). This antibody is specific to ACADM.

Protocols

PRODUCT SPECIFIC PROTOCOLS

STANDARD PROTOCOLS

Click here to view our Standard Protocols

ACADM Monoclonal antibody 0 Publications

Mouse Monoclonal| Catalog number: 67742-1-Ig |CloneNo.: 1A11G6