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ACBD4 Polyclonal antibody

ACBD4 Polyclonal Antibody for IF/ICC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF/ICC, WB,ELISA

Conjugate

Unconjugated

Cat No : 20941-1-AP

Print datasheet

Synonyms

ACBD4, HMFT0700

验证数据展示

  • WB analysis of human kidney using 20941-1-AP

    WB analysis of human kidney using 20941-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 20941-1-AP (ACBD4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 20941-1-AP

    IF Staining of MCF-7 using 20941-1-AP

    Immunofluorescent analysis of MCF-7 cells using 20941-1-AP (ACBD4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inhuman kidney tissue
Positive IF detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 20941-1-AP is ACBD4 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivitymouse
Immunogen ACBD4 fusion protein Ag15045 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name acyl-Coenzyme A binding domain containing 4
Synonyms ACBD4, HMFT0700
Calculated Molecular Weight 341 aa, 38 kDa
Observed Molecular Weight 30-38 kDa
GenBank Accession NumberBC029164
Gene Symbol ACBD4
Gene ID (NCBI) 79777
RRIDAB_2878769
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NC06
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ACBD4 antibody 20941-1-APDownload protocol
IF protocol for ACBD4 antibody 20941-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Struct Mol Biol

TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.

Authors - Xinxin Zuo
View Article