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ACC Monoclonal antibody 3 Publications

Mouse Monoclonal| Catalog number: 67373-1-Ig |CloneNo.: 1A11G10

浓度: 1000 μg/ml

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Applications

Tested Applications:
IHC, WB, ELISA
Application not tested? Please see our Guarantee


Cited Applications:
WB


Images See all

WB analysis using 67373-1-Ig

Various lysates were subjected to SDS PAGE followed by western blot with 67373-1-Ig (ACC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

IHC staining of human breast cancer using 67373-1-Ig

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67373-1-Ig (ACC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IHC staining of human breast cancer using 67373-1-Ig

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67373-1-Ig (ACC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Species specificity:
Human, Mouse, Rat
Positive WB detected in:
HeLa cells, NIH/3T3 cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells
Positive IHC detected in:
human breast cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:10000-1:50000
IHC : 1:500-1:2000

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2a
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ACC fusion protein Ag17503
Full name:
acetyl-Coenzyme A carboxylase alpha
Calculated molecular weight:
2383 aa, 275 kDa
Observed molecular weight:
260-270 kDa
GenBank accession number:
BC137287
Gene ID (NCBI):
31
Gene symbol
ACACA
Synonyms
ACAC, ACACA, ACC, ACC1, ACCA, Acetyl CoA carboxylase 1

Background

ACACA(Acetyl-CoA carboxylase 1, ACC), also named as ACAC, ACC1 and ACCA, belongs to the biotin containing enzyme family. It catalyzes the synthesis of malonyl-CoA, which is an intermediate substrate playing a pivotal role in the regulation of fatty acid metabolism and energy production. ACACA is involved in the biosynthesis of fatty acids, and malonyl-CoA produced is used as a building block to extend the chain length of fatty acids by fatty acid synthase (FAS)(PMID:19900410). It has 4 isoforms produced by alternative promoter usage with the molecular weight between 260 kDa and 270 kDa.

Protocols

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