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ASIC2 Polyclonal antibody

ASIC2 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 17851-1-AP

Print datasheet

Synonyms

ACCN, ACCN1, Acid sensing ion channel 2, ASIC2, ASIC2a, BNaC1, BNC1, Brain sodium channel 1, hBNaC1, Mammalian degenerin homolog, MDEG

验证数据展示

  • WB analysis of rat brain using 17851-1-AP

    WB analysis of rat brain using 17851-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 17851-1-AP (ASIC2 antibody at dilution of 1:300 incubated at 4 degree celsius over night.

  • IP experiment of mouse brain using 17851-1-AP

    IP experiment of mouse brain using 17851-1-AP

    IP result of anti-ASIC2 (IP:17851-1-AP, 4ug; Detection:17851-1-AP 1:500) with mouse brain tissue lysate 4000ug.

经过测试的应用

Positive WB detected inrat brain tissue
Positive IP detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

The immunogen of 17851-1-AP is ASIC2 Fusion Protein expressed in E. coli.

Tested Applications IP, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen ASIC2 fusion protein Ag12301 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name amiloride-sensitive cation channel 1, neuronal
Synonyms ACCN, ACCN1, Acid sensing ion channel 2, ASIC2, ASIC2a, BNaC1, BNC1, Brain sodium channel 1, hBNaC1, Mammalian degenerin homolog, MDEG
Calculated Molecular Weight 512 aa, 58 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC075043
Gene Symbol ACCN1
Gene ID (NCBI) 40
RRIDAB_2878451
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16515
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ASIC2 (acid-sensing ion channel 2), also known as ACCN1 (amiloride-sensitive cation channel 1, neuronal), is a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. ASIC2 is widely expressed in the central nervous system (CNS), may playing a role in neurotransmission. It has two alternatively spliced variants, ASIC2a and ASIC2b.

实验方案

Product Specific Protocols
WB protocol for ASIC2 antibody 17851-1-APDownload protocol
IP protocol for ASIC2 antibody 17851-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Aging (Albany NY)

ASIC1 and ASIC3 mediate cellular senescence of human nucleus pulposus mesenchymal stem cells during intervertebral disc degeneration.

Authors - Jingyu Ding
View Article
humanWB

Sci Rep

Syntabulin regulates the trafficking of PICK1-containing vesicles in neurons.

Authors - Junyu Xu
View Article