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ACE2 Monoclonal antibody 6 Publications

Mouse Monoclonal| Catalog number: 66699-1-Ig |CloneNo.: 2F12A4

浓度: 1000 μg/ml

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Applications

Tested Applications:
IHC, WB, ELISA
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Cited Applications:
FC, IF, WB


Images See all

WB analysis of human testis using 66699-1-Ig

Untreated and PNGase F-treated lysates of human testis tissue were subjected to SDS PAGE followed by western blot with 66699-1-Ig (ACE2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

IHC staining of human small intestine using 66699-1-Ig

Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66699-1-Ig (ACE2 antibody) at dilution of 1:2000 (under 10x lens)..

IHC staining of human small intestine using 66699-1-Ig

Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66699-1-Ig (ACE2 antibody) at dilution of 1:2000 (under 40x lens)..

Species specificity:
Human
Positive WB detected in:
human testis tissue, human skeletal muscle tissue
Positive IHC detected in:
human small intestine tissue, human testis tissue, human kidney tissue, human colon tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:2000-1:6000
IHC : 1:1000-1:4000

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG1
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ACE2 fusion protein Ag15554
Full name:
angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
Calculated molecular weight:
805 aa, 92 kDa
Observed molecular weight:
120 kDa, 92 kDa
GenBank accession number:
BC048094
Gene ID (NCBI):
59272
Gene symbol
ACE2
Synonyms
ACE related carboxypeptidase, ACE2, ACEH, DKFZp434A014, Metalloprotease MPROT15

Background

ACE2 (Angiotensin-converting enzyme 2), also named as ACEH, is a zinc metalloprotease of the ACE family and a critical regulator of the reninangiotensin system. ACE2 has a more restricted tissue distribution than ACE, being found predominantly in the heart, kidneys, and testes although low levels have been detected in a variety of tissues (PMID:15983030). ACE2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 (PMID: 32142651). The expression level and expression pattern of human ACE2 in different tissues might be critical for the susceptibility, symptoms, and outcome of 2019-nCoV/SARS-CoV-2 infection (PMID: 32133153). It can be used as a potential therapeutic target of SARS-CoV-2 (PMID: 32125455). The calculated molecular weight of ACE2 is 92kDa but it migrates to 120kDa due to N-glycosylation (PMID:16166094). Sometimes, several cleaved fragments can also be detected as 75kDa, 50 kDa or 37kDa (PMID: 29561187, 22009550, 30759273). It has 2 isoforms produced by alternative splicing. This antibody is specific to ACE2. The location of ACE2 is membrane and cytoplasm, however it accumulates in the nucleus during the mitosis (PMID: 1730413/PMID: 18292088).

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