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ACE2 Antibody 0 Publications

Mouse Monoclonal| Catalog number: 66699-1-Ig |CloneNo.: 2F12A4

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浓度: 1000μg/ml

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Tested Applications

Applications:
WB, IHC, ELISA
Application not tested? Please see our Guarantee


Images See all

ACE2 Antibody WB human testis tissue 66699-1-Ig

Untreated and PNGase F-treated lysates of human testis tissue were subjected to SDS PAGE followed by western blot with 66699-1-Ig (ACE2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

ACE2 Antibody IHC human small intestine tissue 66699-1-Ig

Immunohistochemistry of paraffin-embedded human small intestine tissue slide using 66699-1-Ig (ACE2 antibody) at dilution of 1:300 (under 10x lens).

ACE2 Antibody IHC human small intestine tissue 66699-1-Ig

Immunohistochemistry of paraffin-embedded human small intestine tissue slide using 66699-1-Ig (ACE2 antibody) at dilution of 1:300 (under 40x lens).

Species specificity:
Human
Positive WB detected in:
human testis tissue, human skeletal muscle tissue
Positive IHC detected in:
human small intestine tissue, human testis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:2000-1:6000
IHC : 1:150-1:600

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ACE2 fusion protein Ag15554
Full name:
angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
Calculated molecular weight:
805aa,92 kDa
Observed molecular weight:
120 kDa, 92 kDa
GenBank accession number:
BC048094
Gene ID (NCBI):
59272
Gene symbol
ACE2
Synonyms
ACE related carboxypeptidase, ACE2, ACEH, DKFZp434A014, Metalloprotease MPROT15

Background

ACE2 contains a potential 17-amino acid N-terminal signal peptide and a putative 22-amino acid C-terminal membrane anchor. It is the only known mammalian homologue of the zinc-metallopeptidase angiotensin-converting enzyme (ACE), a critical regulator of the reninangiotensin system. ACE2 has a more restricted tissue distribution than ACE, being found predominantly in the heart, kidneys, and testes although low levels have been detected in a variety of tissues (PMID:15983030). The calculated molecular weight of ACE2 is 92kDa but it migrates to 120kDa due to N-glycosylation (PMID:16166094). It has 2 isoforms produced by alternative splicing. This antibody is specific to ACE2.


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