Name: ACLY Mouse Monoclonal antibody
Brand: Proteintech

Applications

Tested Applications:

IF, IHC, WB, ELISA
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Jurkat cells were subjected to SDS PAGE followed by western blot with 67166-1-Ig (ACLY antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

IHC staining of human prostate cancer using 67166-1-Ig

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67166-1-Ig (ACLY antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67166-1-Ig (ACLY antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 67166-1-Ig (ACLY antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Species specificity:

Human, Mouse, Rat

Positive WB detected in:

Jurkat cells, MCF-7 cells, HSC-T6 cells

Positive IHC detected in:

human prostate cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Positive IF detected in:

A549 cells

Recommended dilution:

WB : 1:1000-1:10000

IHC : 1:500-1:3000

IF : 1:50-1:500

Product Information

Source:

Mouse

Purification method:

Protein A purification

Isotype:

IgM

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

ACLY fusion protein Ag7709

Full name:

ATP citrate lyase

Calculated molecular weight:

121 kDa

Observed molecular weight:

120 kDa

GenBank accession number:

BC006195

Gene ID (NCBI):

47

Gene symbol

ACLY

Synonyms

ACL, ACLY, ATP citrate (pro S ) lyase, ATP citrate lyase, ATP citrate synthase, ATPCL, Citrate cleavage enzyme, CLATP

Background

ACLY(ATP-citrate synthase) is also named as ACL. It is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA. ACLY serves as not only a target in oxygenated cells for suppression of lipid synthesis and histone acetylation, but also as a susceptible target in hypoxic cells to restore inhibition of glycolysis. In nonsmall cell lung carcinoma and hepatocellular carcinoma, ACLY is overexpressed compared with normal parenchyma suggesting that ACLY may represent a common target among highly malignant tumors(PMID:19795461). This protein has 2 isoforms produced by alternative splicing.