Name: ACO2 Mouse Monoclonal antibody
Brand: Proteintech

Applications

Tested Applications:

IHC, WB, ELISA
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Various lysates were subjected to SDS PAGE followed by western blot with 67509-1-Ig (ACO2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

IHC staining of human liver cancer using 67509-1-Ig

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67509-1-Ig (ACO2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67509-1-Ig (ACO2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Species specificity:

Human, Mouse, Rat, Pig

Positive WB detected in:

pig brain tissue, mouse brain tissue, rat brain tissue, pig cerebellum tissue, rat cerebellum tissue, mouse cerebellum tissue

Positive IHC detected in:

human liver cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution:

WB : 1:5000-1:50000

IHC : 1:500-1:2000

Product Information

Source:

Mouse

Purification method:

Protein G purification

Isotype:

IgG1

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

ACO2 fusion protein Ag17784

Full name:

aconitase 2, mitochondrial

Calculated molecular weight:

85 kDa

Observed molecular weight:

85 kDa

GenBank accession number:

BC014092

Gene ID (NCBI):

50

Gene symbol

ACO2

Synonyms

ACO2, Aconitase, aconitase 2, mitochondrial, ACONM, Citrate hydro lyase

Background

ACO2(aconitate hydratase, mitochondrial) is also named as citrate hydro-lyase and belongs to the aconitase/IPM isomerase family. It plays a key function in cellular energy production, and loss of its activity has a major impact on cellular and organismal survival. Western blot shows two bands of 83 kDa and 40 kDa. The 40 kDa fragment decreases with age and oxidative stress, whereas other fragmentation products with molecular weights between 40 and 83 kDa increased with age and MnSOD(mitochondrial manganese superoxide dismutase) deficiency(PMID:12459471). Defects in ACO2 are the cause of infantile cerebellar-retinal degeneration (ICRD).