ACO2 Monoclonal antibody

ACO2 Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

FC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F1G4

Cat No : 67509-1-Ig

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Synonyms

ACO2, Aconitase, aconitase 2, mitochondrial, ACONM, Citrate hydro lyase



经过测试的应用

Positive WB detected inPC-12 cells, pig brain tissue, rat brain tissue, mouse brain tissue, HEK-293 cells, pig cerebellum tissue, rat cerebellum tissue, mouse cerebellum tissue, Neuro-2a cells, rabbit brain tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ACO2 Ready-To-Use IHC Kit. ACO2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67509-1-Ig targets ACO2 in WB, IHC, FC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications FC, IHC, WB, ELISA
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen ACO2 fusion protein Ag17784 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name aconitase 2, mitochondrial
Synonyms ACO2, Aconitase, aconitase 2, mitochondrial, ACONM, Citrate hydro lyase
Calculated Molecular Weight 85 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberBC014092
Gene Symbol ACO2
Gene ID (NCBI) 50
RRIDAB_2882730
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ99798
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACO2(aconitate hydratase, mitochondrial) is also named as citrate hydro-lyase and belongs to the aconitase/IPM isomerase family. It plays a key function in cellular energy production, and loss of its activity has a major impact on cellular and organismal survival. Western blot shows two bands of 83 kDa and 40 kDa. The 40 kDa fragment decreases with age and oxidative stress, whereas other fragmentation products with molecular weights between 40 and 83 kDa increased with age and MnSOD(mitochondrial manganese superoxide dismutase) deficiency(PMID:12459471). Defects in ACO2 are the cause of infantile cerebellar-retinal degeneration (ICRD).

实验方案

Product Specific Protocols
WB protocol for ACO2 antibody 67509-1-IgDownload protocol
IHC protocol for ACO2 antibody 67509-1-IgDownload protocol
FC protocol for ACO2 antibody 67509-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols