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验证数据展示

  • WB analysis of K-562 using 60176-1-Ig

    WB analysis of K-562 using 60176-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 60176-1-Ig (ACPP Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of human prostate cancer using 60176-1-Ig

    IF Staining of human prostate cancer using 60176-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using ACPP antibody (60176-1-Ig, Clone: 1G2C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human prostate cancer using 60176-1-Ig

    IF Staining of human prostate cancer using 60176-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using ACPP antibody (60176-1-Ig, Clone: 1G2C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of human prostate hyperplasia using 60176-1-Ig

    IHC staining of human prostate hyperplasia using 60176-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 60176-1-Ig (ACPP antibody) at dilution of 1:1000 (under 10x lens).

    Ready-to-use IHC kits available with PAP/ACPP antibody included
  • IHC staining of human prostate hyperplasia using 60176-1-Ig

    IHC staining of human prostate hyperplasia using 60176-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 60176-1-Ig (ACPP antibody) at dilution of 1:1000 (under 40x lens).

    Ready-to-use IHC kits available with PAP/ACPP antibody included

ACPP Monoclonal antibody

ACPP Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 60176-1-Ig

产品说明书

CloneNo. 1G2C9
引用文献(1)

宿主/亚型

Mouse / IgG2a

种属反应性

human, rat

应用

WB, IHC, IF-P, ELISA

别名

PAP, 1G2C9, 5'-NT, 5'-nucleotidase, Acid phosphatase 3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells, human testis tissue
Positive IHC detected inhuman prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman prostate cancer tissue
Planning an IHC experiment? We recommend our IHCeasy PAP/ACPP Ready-To-Use IHC Kit. PAP/ACPP primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

60176-1-Ig targets ACPP in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB
经测试反应性 human, rat
文献引用反应性human
免疫原

CatNo: Ag8578

Product name: Recombinant human ACPP protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 27-373 aa of BC007460

Sequence: DRSVLAKELKFVTLVFRHGDRSPIDTFPTDPIKESSWPQGFGQLTQLGMEQHYELGEYIRKRYRKFLNESYKHEQVYIRSTDVDRTLMSAMTNLAALFPPEGVSIWNPILLWQPIPVHTVPLSEDQLLYLPFRNCPRFQELESETLKSEEFQKRLHPYKDFIATLGKLSGLHGQDLFGIWSKVYDPLYCESVHNFTLPSWATEDTMTKLRELSELSLLSLYGIHKQKEKSRLQGGVLVNEILNHMKRATQIPSYKKLIMYSAHDTTVSGLQMALDVYNGLLPPYASCHLTELYFEKGEYFVEMYYRNETQHEPYPLMLPGCSPSCPLERFAELAGPVIPQDWSTECM

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 acid phosphatase, prostate
别名 PAP, 1G2C9, 5'-NT, 5'-nucleotidase, Acid phosphatase 3
计算分子量 418 aa, 48 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC007460
基因名称 ACPP
Gene ID (NCBI) 55
RRIDAB_10733336
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP15309
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACPP is also named as PAP(prostatic acid phosphatase),5'-nucleotidase,thiamine monophosphatas and Belongs to the histidine acid phosphatase family.This enzyme is a homodimeric glycoprotein with a subunit molecular mass of 50 kDa and is an androgen-stimulated secretory product of the human prostate gland(PMID:8132635).It is also a non-specific phosphatase and has been implicated in the progression of prostate cancer, which inactivates LPA in human seminal plasma(PMID:15280042). This protein has three glycosylation sites(PMID:10639192;12525165).

实验方案

Product Specific Protocols
WB protocol for ACPP antibody 60176-1-IgDownload protocol
IHC protocol for ACPP antibody 60176-1-IgDownload protocol
IF protocol for ACPP antibody 60176-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Endocrinol (Lausanne)

Proteomic Analysis of the Alterations in Follicular Fluid Proteins During Oocyte Maturation in Humans.

Authors - Chong Wang
View Article