验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, human testis tissue |
| Positive IHC detected in | human prostate hyperplasia tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human prostate cancer tissue |
Planning an IHC experiment? We recommend our IHCeasy PAP/ACPP Ready-To-Use IHC Kit. PAP/ACPP primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
60176-1-Ig targets ACPP in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, rat |
| 文献引用反应性 | human |
| 免疫原 | ACPP fusion protein Ag8578 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | acid phosphatase, prostate |
| 别名 | acid phosphatase, prostate, ACP 3, ACP3, ACPP, PAP, Prostatic acid phosphatase |
| 计算分子量 | 418 aa, 48 kDa |
| 观测分子量 | 45-50 kDa |
| GenBank蛋白编号 | BC007460 |
| 基因名称 | ACPP |
| Gene ID (NCBI) | 55 |
| RRID | AB_10733336 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P15309 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ACPP is also named as PAP(prostatic acid phosphatase),5'-nucleotidase,thiamine monophosphatas and Belongs to the histidine acid phosphatase family.This enzyme is a homodimeric glycoprotein with a subunit molecular mass of 50 kDa and is an androgen-stimulated secretory product of the human prostate gland(PMID:8132635).It is also a non-specific phosphatase and has been implicated in the progression of prostate cancer, which inactivates LPA in human seminal plasma(PMID:15280042). This protein has three glycosylation sites(PMID:10639192;12525165).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ACPP antibody 60176-1-Ig | Download protocol |
| IHC protocol for ACPP antibody 60176-1-Ig | Download protocol |
| IF protocol for ACPP antibody 60176-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
