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AFM Monoclonal antibody, PBS Only

AFM Monoclonal Antibody for WB,IP,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB,IP,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

2G9F6

Cat No : 66113-1-PBS

Print datasheet

Synonyms

afamin, afamin/AFM, AFM, ALB2, ALBA, ALF, Alpha Alb, Alpha albumin

验证数据展示

  • IP experiment of human plasma using 66113-1-Ig (same clone as 66113-1-PBS)

    IP experiment of human plasma using 66113-1-Ig (same clone as 66113-1-PBS)

    IP result of anti-AFM (IP:66113-1-Ig, 5ug; Detection:66113-1-Ig 1:500) with human plasma lysate 4000ug. This data was developed using the same antibody clone with 66113-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66113-1-Ig (same clone as 66113-1-PBS)

    IHC staining of human liver using 66113-1-Ig (same clone as 66113-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66113-1-Ig (AFM Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66113-1-PBS in a different storage buffer formulation.

  • WB analysis of human plasma using 66113-1-Ig (same clone as 66113-1-PBS)

    WB analysis of human plasma using 66113-1-Ig (same clone as 66113-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66113-1-Ig (AFM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66113-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66113-1-Ig (same clone as 66113-1-PBS)

    IF Staining of HeLa using 66113-1-Ig (same clone as 66113-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using AFM antibody (66113-1-Ig, Clone: 2G9F6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66113-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66113-1-Ig (same clone as 66113-1-PBS)

    IF Staining of HeLa using 66113-1-Ig (same clone as 66113-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AFM antibody (66113-1-Ig, Clone: 2G9F6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66113-1-PBS in a different storage buffer formulation.

产品信息

66113-1-PBS targets AFM in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB,IP,Indirect ELISA,IHC,IF
Tested Reactivity human, mouse
Immunogen AFM fusion protein Ag16866 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name afamin
Synonyms afamin, afamin/AFM, AFM, ALB2, ALBA, ALF, Alpha Alb, Alpha albumin
Calculated Molecular Weight 599 aa, 69 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberBC109020
Gene Symbol AFM
Gene ID (NCBI) 173
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP43652
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

AFM (Afamin) is a member of the albumin superfamily, which comprises albumin, vitamin D-binding protein, a-fetoprotein, and afamin. AFM is present in the plasma/serum, cerebrospinal fluid, and follicular fluid, and functions as a vitamin E-binding protein. Alterations of expression level of AFM have been linked to variety of diseases like cancer.