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AGFG2 Polyclonal antibody

AGFG2 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 11919-1-AP

Print datasheet

Synonyms

AGFG2, ArfGAP with FG repeats 2, HRBL, RAB R, RABR

验证数据展示

  • WB analysis of Jurkat using 11919-1-AP

    WB analysis of Jurkat using 11919-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11919-1-AP (AGFG2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 11919-1-AP

    IHC staining of human lymphoma using 11919-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 11919-1-AP (AGFG2 antibody) at dilution of 1:50 (under 10x lens).

  • IF Staining of HepG2 using 11919-1-AP

    IF Staining of HepG2 using 11919-1-AP

    Immunofluorescent analysis of HepG2 cells, using AGFG2 antibody 11919-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inJurkat cells, K-562 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11919-1-AP targets AGFG2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen AGFG2 fusion protein Ag2546 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ArfGAP with FG repeats 2
Synonyms AGFG2, ArfGAP with FG repeats 2, HRBL, RAB R, RABR
Calculated Molecular Weight 481 aa, 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC017329
Gene Symbol AGFG2
Gene ID (NCBI) 3268
RRIDAB_2224646
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95081
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for AGFG2 antibody 11919-1-APDownload protocol
IHC protocol for AGFG2 antibody 11919-1-APDownload protocol
IF protocol for AGFG2 antibody 11919-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols