AK3 Polyclonal antibody

AK3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 12562-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

AKL3L1, AKL3L, AK6, AK3L1, AK 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inhuman placenta tissue, BxPC-3 cells, HEK-293 cells, HepG2 cells, human kidney tissue, L02 cells, MCF-7 cells, mouse heart tissue, mouse kidney tissue, Y79 cells
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman pancreas cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

12562-1-AP targets AK3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 AK3 fusion protein Ag3146 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 adenylate kinase 3
别名 AKL3L1, AKL3L, AK6, AK3L1, AK 3
计算分子量 227 aa, 26 kDa
观测分子量 26 kDa
GenBank蛋白编号BC013771
基因名称 AK3
Gene ID (NCBI) 50808
RRIDAB_2305373
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UIJ7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AK3(Adenylate kinase 3) is also named as AK3L1, AK6, AKL3L and belongs to the adenylate kinase family. It is located in the mitochondrial matrix and is a GTP:AMP phosphotransferase(PMID:2546792). AK3 can be detected AK3 at an apparent molecular mass of 28 kD by western blot analysis and subcellular fractionation of mouse liver and kidney detected Ak3 in the mitochondrial fraction(PMID:11485571). This antibody may also recognize AK4 due to the high homology.

实验方案

Product Specific Protocols
WB protocol for AK3 antibody 12562-1-APDownload protocol
IHC protocol for AK3 antibody 12562-1-APDownload protocol
IF protocol for AK3 antibody 12562-1-APDownload protocol
IP protocol for AK3 antibody 12562-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Med Rep

MicroRNA‑214 upregulates HIF‑1α and VEGF by targeting ING4 in lung cancer cells.

Authors - Yue Li
humanWB

J Gene Med

MiR-96-5p promotes breast cancer migration by activating MEK/ERK signaling.

Authors - Wei-Yan Qin
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