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AKT Polyclonal antibody 333 Publications

Rabbit Polyclonal| Catalog number: 10176-2-AP

Featured Product KD/KO validated

浓度: 400 μg/ml

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
Application not tested? Please see our Guarantee


Images See all

WB analysis of MCF-7 using 10176-2-AP

WB result of AKT antibody (10176-2-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKT transfected MCF-7 cells.

WB analysis using 10176-2-AP

Various lysates were subjected to SDS PAGE followed by western blot with 10176-2-AP (AKT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

WB analysis using 10176-2-AP

Various lysates were subjected to SDS PAGE followed by western blot with 10176-2-AP (AKT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

WB analysis of HeLa cells using 10176-2-AP

siRNA AKT1 result from Dr. Eva Martinez-Balibrea. Green:tubulin, Red:10176-2-AP, AKT1.

IP experiment of HeLa using 10176-2-AP

IP Result of anti-AKT1 (IP:10176-2-AP, 3ug; Detection:10176-2-AP 1:500) with HeLa cells lysate 2500ug.

IHC staining of human ovary tumor using 10176-2-AP

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10176-2-AP (AKT1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IHC staining of human ovary tumor using 10176-2-AP

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10176-2-AP (AKT1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IF Staining of HeLa using 10176-2-AP

Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 10176-2-AP (AKT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

FC experiment of HeLa using 10176-2-AP

1X10^6 HeLa cells were stained with 0.2ug AKT1 antibody (10176-2-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human, mouse, rat, A. sphaerocephala Krasch, blunt snout bream, carp, chicken, cow, hamster, swine
Positive WB detected in:
HeLa cells, A549 cells, HEK-293 cells, HepG2 cells, human liver tissue, MCF-7 cells, mouse testis tissue, NIH/3T3 cells, C6 cells
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human ovary tumor tissue, human cervical cancer tissue, human breast cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:1000-1:6000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:200-1:1000
IF : 1:50-1:500

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
AKT fusion protein Ag0213
Full name:
v-akt murine thymoma viral oncogene homolog 1
Calculated molecular weight:
56 kDa
Observed molecular weight:
56-62 kDa
GenBank accession number:
BC000479
Gene ID (NCBI):
207
Gene symbol
AKT1
Synonyms
AKT, AKT1, AKT2, AKT3, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha

Background

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.


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