A549 cells were subjected to SDS PAGE followed by western blot with 10176-2-AP (AKT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

A549 cells were subjected to SDS PAGE followed by western blot with 10176-2-AP(AKT1 antibody) at dilution of 1:500.

HEK-293 cells were subjected to SDS PAGE followed by western blot with 10176-2-AP (AKT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 10176-2-AP(AKT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 10176-2-AP(AKT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

siRNA AKT1 result from Dr. Eva Martinez-Balibrea. Green:tubulin, Red:10176-2-AP, AKT1.

Various lysates were subjected to SDS PAGE followed by western blot with 10176-2-AP (AKT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

human liver tissue were subjected to SDS PAGE followed by western blot with 10176-2-AP(AKT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

mouse testis tissue were subjected to SDS PAGE followed by western blot with 10176-2-AP(AKT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

Immunohistochemical of paraffin-embedded human breast cancer using 10176-2-AP(AKT1 antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human breast cancer using 10176-2-AP(AKT1 antibody) at dilution of 1:50 (under 40x lens)

Immunohistochemical of paraffin-embedded human cervical cancer using 10176-2-AP(AKT1 antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human cervical cancer using 10176-2-AP(AKT1 antibody) at dilution of 1:50 (under 40x lens)

Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using 10176-2-AP( AKT1 antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using 10176-2-AP( AKT1 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

IP Result of anti-AKT1 (IP:10176-2-AP, 3ug; Detection:10176-2-AP 1:500) with HeLa cells lysate 2500ug.

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 10176-2-AP(AKT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

1X10^6 HeLa cells were stained with 0.2ug AKT1 antibody (10176-2-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.