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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated AMPK Alpha Monoclonal antibody

AMPK Alpha Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse and More (1)

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H8E1

Cat No : CL488-66536

Print datasheet

Synonyms

AMPK, AMPK alpha, AMPK subunit alpha 1, AMPK1, AMPKa1, PRKAA1

验证数据展示

  • IF Staining of human breast cancer using CL488-66536

    IF Staining of human breast cancer using CL488-66536

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CL488-66536 (AMPK alpha antibody) at dilution of 1:100.

  • IF Staining of human breast cancer using CL488-66536

    IF Staining of human breast cancer using CL488-66536

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CL488-66536 (AMPK alpha antibody) at dilution of 1:100.

经过测试的应用

Positive IF detected inhuman breast cancer tissue
Planning an IHC experiment? We recommend our IHCeasy PRKAA1 Ready-To-Use IHC Kit. PRKAA1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66536-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

CL488-66536 targets AMPK Alpha in IF applications and shows reactivity with Human, Rat, Mouse samples.

Tested Applications IF/ICC
Cited Applications IF
Tested Reactivity Human, Rat, Mouse
Cited Reactivitymouse
Immunogen AMPK Alpha fusion protein Ag24744 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name protein kinase, AMP-activated, alpha 1 catalytic subunit
Synonyms AMPK, AMPK alpha, AMPK subunit alpha 1, AMPK1, AMPKa1, PRKAA1
Calculated Molecular Weight 63 kDa
Observed Molecular Weight 64 kDa
GenBank Accession NumberBC012622
Gene Symbol PRKAA1
Gene ID (NCBI) 5562
RRIDAB_2883352
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13131
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The mammalian 5-prime-AMP-activated protein kinase (AMPK) appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. PRKAA1 is also named as AMPK1, ACACA kinase, HMGCR kinase. It is a mammalian homologue of sucrose non-fermenting protein kinase (SNF-1), which belongs to a serine/threonine protein kinase family. It has 2 isoforms with molecular mass of 63-66 kDa produced by alternative splicing.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 AMPK Alpha antibody CL488-66536Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Cell Rep

Downregulation of hepatic ceruloplasmin ameliorates NAFLD via SCO1-AMPK-LKB1 complex

Authors - Liping Xie
View Article