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  • KD/KO Validated

Annexin A11 Polyclonal antibody

Annexin A11 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 10479-2-AP

Print datasheet

Synonyms

ANXA11, ANX11, Annexin-11, Annexin XI, Annexin 11



经过测试的应用

Positive WB detected inA431 cells, HeLa cells, mouse uterus tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue, human breast cancer tissue, human kidney tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ANXA11 Ready-To-Use IHC Kit. ANXA11 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10479-2-AP targets Annexin A11 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen Annexin A11 fusion protein Ag0729 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name annexin A11
Synonyms ANXA11, ANX11, Annexin-11, Annexin XI, Annexin 11
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 56-60 kDa
GenBank Accession NumberBC007564
Gene Symbol Annexin A11
Gene ID (NCBI) 311
RRIDAB_2057171
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP50995
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Annexin A11 (Anxa11) is one member of annexins that are Ca2+-regulated phospholipid-binding proteins. Annexin A11 plays an important role in cell division, differentiation, apoptosis, vesicle trafficking and Ca2+ signaling. Annexin A11 dysregulation is involved in the progression, drug-resistance, recurrence of systemic autoimmune disease and cancer.

实验方案

Product Specific Protocols
WB protocol for Annexin A11 antibody 10479-2-APDownload protocol
IHC protocol for Annexin A11 antibody 10479-2-APDownload protocol
IF protocol for Annexin A11 antibody 10479-2-APDownload protocol
IP protocol for Annexin A11 antibody 10479-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC,IF

Sci Transl Med

Mutations in the vesicular trafficking protein annexin A11 are associated with amyotrophic lateral sclerosis.

Authors - Bradley N Smith
humanIHC,IF

Ann Neurol

A Novel Multisystem Proteinopathy Caused by a Missense ANXA11 Variant.

Authors - Tauana Bernardes Leoni
mouseWB

Oncotarget

ANXA11 regulates the tumorigenesis, lymph node metastasis and 5-fluorouracil sensitivity of murine hepatocarcinoma Hca-P cells by targeting c-Jun.

Authors - Shuqing Liu
  • KD Validated
mouseWB

Biomed Pharmacother

Annexin A11 knockdown inhibits in vitro proliferation and enhances survival of Hca-F cell via Akt2/FoxO1 pathway and MMP-9 expression.

Authors - Shuqing Liu
  • KD Validated
humanWB,IHC

Neurobiol Aging

Genetic screening of ANXA11 revealed novel mutations linked to amyotrophic lateral sclerosis.

Authors - Elisa Teyssou
humanIHC

Ann Clin Transl Neurol

Adult-onset dominant muscular dystrophy in Greek families caused by Annexin A11

Authors - Mridul Johari