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APH1A Polyclonal antibody

APH1A Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 11643-1-AP

Print datasheet

Synonyms

6530402N02Rik, APH 1A, Aph 1alpha, APH1A, CGI 78, Gamma secretase subunit APH 1A, PSF, UNQ579/PRO1141

验证数据展示

  • WB analysis using 11643-1-AP

    WB analysis using 11643-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11643-1-AP (APH1A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 11643-1-AP

    IP experiment of HeLa using 11643-1-AP

    IP result of anti-APH1A (IP:11643-1-AP, 4ug; Detection:11643-1-AP 1:4000) with HeLa cells lysate 1600 ug.

经过测试的应用

Positive WB detected inHT-29 cells, HeLa cells, human brain tissue, U-937 cells, rat brain tissue
Positive IP detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

11643-1-AP targets APH1A in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Applications IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen APH1A fusion protein Ag2238 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name anterior pharynx defective 1 homolog A (C. elegans)
Synonyms 6530402N02Rik, APH 1A, Aph 1alpha, APH1A, CGI 78, Gamma secretase subunit APH 1A, PSF, UNQ579/PRO1141
Calculated Molecular Weight 265 aa, 29 kDa
Observed Molecular Weight27-32 kDa
GenBank Accession NumberBC015568
Gene Symbol APH1A
Gene ID (NCBI) 51107
RRIDAB_2242861
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96BI3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for APH1A antibody 11643-1-APDownload protocol
IP protocol for APH1A antibody 11643-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Neurobiol

Memantine, a Noncompetitive N-Methyl-D-Aspartate Receptor Antagonist, Attenuates Cerebral Amyloid Angiopathy by Increasing Insulin-Degrading Enzyme Expression.

Authors - Yasuteru Inoue
View Article
humanWB

Front Neurosci

Impacts of Acute Hypoxia on Alzheimer's Disease-Like Pathologies in APPswe/PS1dE9 Mice and Their Wild Type Littermates.

Authors - Feng Zhang
View Article
mouseWB

Neurosci Lett

Metformin attenuates vascular pathology by increasing expression of insulin-degrading enzyme in a mixed model of cerebral amyloid angiopathy and type 2 diabetes mellitus.

Authors - Yasuteru Inoue
View Article