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CoraLite® Plus 488-conjugated Arginase-1 Polyclonal antibody

Arginase-1 Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-16001

Print datasheet

Synonyms

ARG1, Arginase 1, arginase, liver, Liver type arginase, Type I arginase

验证数据展示

  • IF Staining of human liver cancer using CL488-16001

    IF Staining of human liver cancer using CL488-16001

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 488 ARG1 antibody (CL488-16001) at dilution of 1:400.

经过测试的应用

Positive IF detected inhuman liver cancer tissue
Planning an IHC experiment? We recommend our IHCeasy ARG1 Ready-To-Use IHC Kit. ARG1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 16001-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-16001 targets Arginase-1 in IF applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P
Tested Reactivity human, mouse, rat
Immunogen Arginase-1 fusion protein Ag8595 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name arginase, liver
Synonyms ARG1, Arginase 1, arginase, liver, Liver type arginase, Type I arginase
Calculated Molecular Weight236aa,25 kDa; 322aa,35 kDa
Observed Molecular Weight 35-36 kDa
GenBank Accession NumberBC005321
Gene Symbol ARG1
Gene ID (NCBI) 383
RRIDAB_2919127
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP05089
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Arginase-1 (Liver arginase) belongs to the arginase family. ARG1 is a novel immunohistochemical marker of hepatocellular differentiation in fine needle aspiration cytology and a marker of hepatocytes and hepatocellular neoplasms. ARG1 is closely associated with alternative macrophage activation(PMID:12098359) and ARG1 has been shown to protect motor neurons from trophic factor deprivation and allow sensory neurons to overcome neurite outgrowth inhibition by myelin proteins(PMID: 20071539). It can exist as a homotrimer(PMID:16141327) and it has 3 isoforms produced by alternative splicing. Defects in ARG1 are the cause of argininemia (ARGIN). Deletion or TNF-mediated restriction of ARG1 unleashes the production of NO by NOS2, which is critical for pathogen control.(PMID:27117406). ARG1 has 3 isoforms with the molecular mass of 25, 35 and 36 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Arginase-1 antibody CL488-16001Download protocol
Standard Protocols
Click here to view our Standard Protocols