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ARMC10 Polyclonal antibody

ARMC10 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 20506-1-AP

Print datasheet

Synonyms

armadillo repeat containing 10, ARMC10, PNAS 112, SVH

验证数据展示

  • IF Staining of HepG2 using 20506-1-AP

    IF Staining of HepG2 using 20506-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 20506-1-AP (ARMC10 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis of A431 using 20506-1-AP

    WB analysis of A431 using 20506-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 20506-1-AP (ARMC10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 20506-1-AP

    IHC staining of human placenta using 20506-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 20506-1-AP (ARMC10 antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of A431 using 20506-1-AP

    IP experiment of A431 using 20506-1-AP

    IP result of anti-ARMC10 (IP:20506-1-AP, 4ug; Detection:20506-1-AP 1:500) with A431 cells lysate 1600ug.

经过测试的应用

Positive WB detected inA431 cells, HEK-293 cells, HepG2 cells, mouse brain tissue, mouse liver tissue
Positive IP detected inA431 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, A431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 20506-1-AP is ARMC10 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen ARMC10 fusion protein Ag14313 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name armadillo repeat containing 10
Synonyms armadillo repeat containing 10, ARMC10, PNAS 112, SVH
Calculated Molecular Weight 343 aa, 38 kDa
Observed Molecular Weight 31-37 kDa
GenBank Accession NumberBC003586
Gene Symbol ARMC10
Gene ID (NCBI) 83787
RRIDAB_10696176
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N2F6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ARMC10 antibody 20506-1-APDownload protocol
IHC protocol for ARMC10 antibody 20506-1-APDownload protocol
IF protocol for ARMC10 antibody 20506-1-APDownload protocol
IP protocol for ARMC10 antibody 20506-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

Cell Death Dis

The Armc10/SVH gene: genome context, regulation of mitochondrial dynamics and protection against Aβ-induced mitochondrial fragmentation.

Authors - R Serrat
View Article
humanWB

Heliyon

Prognostic value and immune infiltration of ARMC10 in pancreatic adenocarcinoma via integrated bioinformatics analyses

Authors - Tian-Hao Li
  • KD Validated
View Article