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ASAH1 Monoclonal antibody

ASAH1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig and More (1)

Applications

IF, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

1B7E4

Cat No : 67092-1-Ig

Print datasheet

Synonyms

AC, Acid ceramidase, Acylsphingosine deacylase, ASAH, ASAH1, FLJ21558, FLJ22079, HSD 33, PHP, PHP32, Putative 32 kDa heart protein



经过测试的应用

Positive WB detected inrat cerebellum tissue, rat brain tissue, mouse brain tissue, pig cerebellum tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy ASAH1 Ready-To-Use IHC Kit. ASAH1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67092-1-Ig targets ASAH1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications IF, IHC, WB, ELISA
Cited ApplicationsIF, IP, WB
Tested Reactivity Human, Mouse, Rat, Pig
Cited Reactivitymouse
Immunogen ASAH1 fusion protein Ag28645 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name N-acylsphingosine amidohydrolase (acid ceramidase) 1
Synonyms AC, Acid ceramidase, Acylsphingosine deacylase, ASAH, ASAH1, FLJ21558, FLJ22079, HSD 33, PHP, PHP32, Putative 32 kDa heart protein
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC016828
Gene Symbol ASAH1
Gene ID (NCBI) 427
RRIDAB_2882397
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13510
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ASAH1, also named as AC, ACDase, Acid CDase, PHP32 and ASAH, belongs to the acid ceramidase family. ASAH1 is a lipid hydrolase that catalyzes the conversion of ceramide (cer) into sphingosine (SPH) and a free fatty acid. Mutation of ASAH1 will cause the Farber lipogranulomatosis (FL) which also known as Farber disease (FD). ASAH1 is highly expressed in heart. And it is first synthesized as an 55-60 kDa precursor and subsequently processed to the mature, heterodimeric enzyme (40 + 13 kDa) in the endosomes/lysosomes.

实验方案

Product Specific Protocols
WB protocol for ASAH1 antibody 67092-1-IgDownload protocol
IHC protocol for ASAH1 antibody 67092-1-IgDownload protocol
IF protocol for ASAH1 antibody 67092-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF,IP

Mol Med

Acid ceramidase targeting pyruvate kinase affected trypsinogen activation in acute pancreatitis

Authors - Juan Xiao
  • KD Validated