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  • Featured Product
  • KD/KO Validated

ASF1B-specific Polyclonal antibody

ASF1B-specific Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 22258-1-AP

Print datasheet

Synonyms

ASF1B, ASF1B-specific, CCG1 interacting factor A II, CIA II, hAsf1, hAsf1b, hCIA II, Histone chaperone ASF1B

验证数据展示

  • WB analysis using 22258-1-AP

    WB analysis using 22258-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22258-1-AP (ASF1B-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 22258-1-AP

    IHC staining of human lymphoma using 22258-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 22258-1-AP (ASF1B-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ASF1B antibody included
  • IF Staining of HeLa using 22258-1-AP

    IF Staining of HeLa using 22258-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 22258-1-AP (ASF1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA431 cells, HEK-293 cells, HeLa cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ASF1B Ready-To-Use IHC Kit. ASF1B primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22258-1-AP targets ASF1B-specific in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen ASF1B-specific fusion protein Ag17763 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ASF1 anti-silencing function 1 homolog B (S. cerevisiae)
Synonyms ASF1B, ASF1B-specific, CCG1 interacting factor A II, CIA II, hAsf1, hAsf1b, hCIA II, Histone chaperone ASF1B
Calculated Molecular Weight 202 aa, 22 kDa
Observed Molecular Weight 20-22 kDa
GenBank Accession NumberBC010014
Gene Symbol ASF1B
Gene ID (NCBI) 55723
RRIDAB_2879051
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NVP2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ASF1B is a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly. This antibody specifically reacts with the 19kd ASF1B protein.

实验方案

Product Specific Protocols
WB protocol for ASF1B-specific antibody 22258-1-APDownload protocol
IHC protocol for ASF1B-specific antibody 22258-1-APDownload protocol
IF protocol for ASF1B-specific antibody 22258-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

RIF1-ASF1-mediated high-order chromatin structure safeguards genome integrity.

Authors - Sumin Feng
  • KD Validated
View Article
mouseWB,IF

Epigenetics Chromatin

Distinct role of histone chaperone Asf1a and Asf1b during fertilization and pre-implantation embryonic development in mice.

Authors - Xuemei Wang
View Article