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ATG101 Polyclonal antibody

ATG101 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 26562-1-AP

Print datasheet

Synonyms

ATG101, Autophagy related protein 101, C12orf44

验证数据展示

  • WB analysis of mouse brain using 26562-1-AP

    WB analysis of mouse brain using 26562-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 26562-1-AP (ATG101 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 26562-1-AP

    IHC staining of human liver cancer using 26562-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26562-1-AP (ATG101 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of HeLa using 26562-1-AP

    IP experiment of HeLa using 26562-1-AP

    IP result of anti-ATG101 (IP:26562-1-AP, 4ug; Detection:26562-1-AP 1:300) with HeLa cells lysate 2000ug.

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:800
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 26562-1-AP is ATG101 Fusion Protein expressed in E. coli.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen ATG101 fusion protein Ag22932 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 12 open reading frame 44
Synonyms ATG101, Autophagy related protein 101, C12orf44
Calculated Molecular Weight 218 aa, 25 kDa
Observed Molecular Weight 25-28 kDa
GenBank Accession NumberBC005151
Gene Symbol C12orf44
Gene ID (NCBI) 60673
RRIDAB_2880554
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BSB4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ATG101 antibody 26562-1-APDownload protocol
IHC protocol for ATG101 antibody 26562-1-APDownload protocol
IP protocol for ATG101 antibody 26562-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Biol Int

TFAM knockdown undermines SQSTM1 mRNA stability but retards autophagy flux and inhibits tumor cells proliferation under starvation conditions

Authors - Xu Jiang
View Article