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CoraLite® Plus 488-conjugated ATG12 Polyclonal antibody

ATG12 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-11264

Print datasheet

Synonyms

APG12, APG12 like, APG12L, ATG12, Autophagy related protein 12, FBR93, HAPG12, Ubiquitin like protein ATG12

验证数据展示

  • IF Staining of PC-3 using CL488-11264

    IF Staining of PC-3 using CL488-11264

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using CoraLite® Plus 488 ATG12 antibody (CL488-11264) at dilution of 1:200.

经过测试的应用

Positive IF detected inPC-3 cells
For other applications, we recommend the unconjugated version of this antibody, 11264-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11264 targets ATG12 in IF applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC
Tested Reactivity human, mouse, rat
Immunogen ATG12 fusion protein Ag1791 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATG12 autophagy related 12 homolog (S. cerevisiae)
Synonyms APG12, APG12 like, APG12L, ATG12, Autophagy related protein 12, FBR93, HAPG12, Ubiquitin like protein ATG12
Calculated Molecular Weight 15 kDa
Observed Molecular Weight 48-55 kDa
GenBank Accession NumberBC012266
Gene Symbol ATG12
Gene ID (NCBI) 9140
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen Affinity Purified
UNIPROT IDO94817
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATG12, also named as APG12 and APG12L, belongs to the ATG12 family. It is required for autophagy. Free ATG12 is 15kd or 8kd. Conjugated to ATG5, ATG12-ATG5 show 48-55kd.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ATG12 antibody CL488-11264Download protocol
Standard Protocols
Click here to view our Standard Protocols