验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
| Positive IHC detected in | human pancreas cancer tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25155-1-AP targets ATG2B in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | ATG2B fusion protein Ag18412 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ATG2 autophagy related 2 homolog B (S. cerevisiae) |
| 别名 | C14orf103, Autophagy-related protein 2 homolog B |
| 计算分子量 | 2078 aa, 233 kDa |
| 观测分子量 | 240-250 kDa |
| GenBank蛋白编号 | BC015016 |
| 基因名称 | ATG2B |
| Gene ID (NCBI) | 55102 |
| RRID | AB_2810874 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96BY7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ATG2B antibody 25155-1-AP | Download protocol |
| IHC protocol for ATG2B antibody 25155-1-AP | Download protocol |
| IF protocol for ATG2B antibody 25155-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol ATG9A protects the plasma membrane from programmed and incidental permeabilization.
| ||
Nat Commun The autophagy protein ATG9A enables lipid mobilization from lipid droplets.
| ||
Autophagy SHISA5/SCOTIN restrains spontaneous autophagy induction by blocking contact between the ERES and phagophores. | ||
Autophagy Architecture of the ATG2B-WDR45 complex and an aromatic Y/HF motif crucial for complex formation. | ||
EMBO Rep A conserved ATG2-GABARAP family interaction is critical for phagophore formation. |
