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ATG5 Polyclonal antibody 100 Publications

Rabbit Polyclonal| Catalog number: 10181-2-AP

Featured Product KD/KO validated

浓度: 300 μg/ml

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Applications

Tested Applications:
IF, IHC, WB,ELISA
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Cited Applications:
FC, IF, IHC, WB


Images See all

WB analysis using 10181-2-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 10181-2-AP (ATG5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

WB analysis using 10181-2-AP

Various lysates were subjected to SDS PAGE followed by western blot with 10181-2-AP (ATG5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

WB analysis of SH-SY5Y using 10181-2-AP

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10181-2-AP (ATG5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

WB analysis of HepG2 using 10181-2-AP

WB result of ATG5 antibody (10181-2-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG5 transfected HepG2 cells.

IF Staining of mouse cortical neurons using 10181-2-AP

IF result of anti-ATG5 (10181-2-AP) with E15 mouse cortical neurons.

IHC staining of human colon cancer using 10181-2-AP

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10181-2-AP (ATG5 antibody) at dilution of 1:200 (under 10x lens).

IHC staining of human colon cancer using 10181-2-AP

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10181-2-AP (ATG5 antibody) at dilution of 1:200 (under 40x lens).

Species specificity:
human, mouse, rat, grouper, monkey, swine
Positive WB detected in:
A549 cells, MDA-MB-231 cells, T-47D cells, Raji cells, HeLa cells, mouse kidney tissue, human brain tissue, HepG2 cells, SH-SY5Y cells
Positive IHC detected in:
human colon cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
mouse cortical neurons
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:50-1:500
IF : 1:20-1:200

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ATG5 fusion protein Ag0214
Full name:
ATG5 autophagy related 5 homolog (S. cerevisiae)
Calculated molecular weight:
32 kDa
Observed molecular weight:
32 kDa, 40-45 kDa, 50-55 kDa
GenBank accession number:
BC002699
Gene ID (NCBI):
9474
Gene symbol
ATG5
Synonyms
APG5, APG5 LIKE, APG5L, Apoptosis specific protein, ASP, ATG5, Autophagy protein 5, hAPG5

Background

ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection. This antibody is raised against 28-275 amino acids of human ATG5. It can recognize the ATG5-ATG12 complex (55 kDa) which can be truncated and generate a 40-45 kDa band. 10181-2-AP also recognizes the free ATG5 (32 kDa).

Protocols

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