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CoraLite®594-conjugated ATG5 Polyclonal antibody
ATG5 Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
验证数据展示
经过测试的应用
Positive FC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
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Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-10181 targets ATG5 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC (Intra) |
Tested Reactivity | human, mouse, rat |
Immunogen | ATG5 fusion protein Ag0214 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | ATG5 autophagy related 5 homolog (S. cerevisiae) |
Synonyms | APG5, APG5 LIKE, APG5L, Apoptosis specific protein, ASP, ATG5, Autophagy protein 5, hAPG5 |
Calculated Molecular Weight | 32 kDa |
Observed Molecular Weight | 32 kDa, 40-45 kDa, 50-55 kDa |
GenBank Accession Number | BC002699 |
Gene Symbol | ATG5 |
Gene ID (NCBI) | 9474 |
RRID | AB_2919723 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9H1Y0 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection. This antibody is raised against 28-275 amino acids of human ATG5. It can recognize the ATG5-ATG12 complex (55 kDa) which can be truncated and generate a 40-45 kDa band. 10181-2-AP also recognizes the free ATG5 (32 kDa).
实验方案
Product Specific Protocols | |
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FC protocol for CL594 ATG5 antibody CL594-10181 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |