验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, A549 cells, HeLa cells, Jurkat cells, HepG2 cells, SH-SY5Y cells |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16688-1-AP targets ATL2 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, monkey |
| 免疫原 | ATL2 fusion protein Ag10030 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | atlastin GTPase 2 |
| 别名 | ARL-6-interacting protein 2, ARL3IP2, ARL 6 interacting protein 2, Aip-2, Aip 2 |
| 计算分子量 | 412aa,47 kDa; 583aa,66 kDa |
| 观测分子量 | 66 kDa |
| GenBank蛋白编号 | BC053508 |
| 基因名称 | ATL2 |
| Gene ID (NCBI) | 64225 |
| RRID | AB_1850898 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8NHH9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATL2, also known as Atlastin GTPase 2, is involved in various cellular processes, including the organization of the Golgi apparatus, the endoplasmic reticulum tubular network membrane organization, and protein homooligomerization. It is located in the endoplasmic reticulum tubular network membrane and is an integral component of the membrane. The ATL2 gene has multiple transcripts and isoforms, with 18 transcripts (splice variants), 297 orthologues, and 10 paralogues. ATL2 also plays a significant role in the immune response, particularly in plants. In Arabidopsis thaliana, the ATL2 gene is involved in the response to fungal pathogen infection. The expression of ATL2 is low under normal growth conditions but is rapidly and significantly induced by exogenous chitin.also plays
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ATL2 antibody 16688-1-AP | Download protocol |
| IHC protocol for ATL2 antibody 16688-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Struct Mol Biol Aurora kinase A-mediated phosphorylation triggers structural alteration of Rab1A to enhance ER complexity during mitosis | ||
J Cell Biol Atlastin 2/3 regulate ER targeting of the ULK1 complex to initiate autophagy. | ||
J Biochem Lunapark ubiquitinates atlastin-2 for the tubular network formation of the endoplasmic reticulum. | ||
NPJ Parkinsons Dis Deficiency of Perry syndrome-associated p150Glued in midbrain dopaminergic neurons leads to progressive neurodegeneration and endoplasmic reticulum abnormalities |
