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验证数据展示

  • WB analysis using 66673-1-Ig

    WB analysis using 66673-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66673-1-Ig (ATP5D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 66673-1-Ig

    IHC staining of human kidney using 66673-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66673-1-Ig (ATP5D antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATP5D antibody included
  • IHC staining of human kidney using 66673-1-Ig

    IHC staining of human kidney using 66673-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66673-1-Ig (ATP5D antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATP5D antibody included

ATP5D Monoclonal antibody

ATP5D Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66673-1-Ig

产品说明书

CloneNo. 1G12C7

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IHC, ELISA

别名

ATP5D, F ATPase delta subunit

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ATP5D Ready-To-Use IHC Kit. ATP5D primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66673-1-Ig targets ATP5D in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human
免疫原 ATP5D fusion protein Ag7016 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit
别名 ATP5D, F ATPase delta subunit
计算分子量 17 kDa
观测分子量 15-17 kDa
GenBank蛋白编号BC002389
基因名称 ATP5D
Gene ID (NCBI) 513
RRIDAB_2882027
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP30049
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The mitochondrial F1Fo-ATP synthase complex uses energy derived from a proton gradient to synthesize ATP. The ATP5D gene encodes the delta subunit of the mitochondrial ATP synthase F1 complex and belongs to the ATPase epsilon chain family. This gene encode the full length protein of 17 kDa with a transit peptide of 22 amino acid.

实验方案

Product Specific Protocols
WB protocol for ATP5D antibody 66673-1-IgDownload protocol
IHC protocol for ATP5D antibody 66673-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols