验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells |
Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66673-1-Ig targets ATP5D in WB, IHC, ELISA applications and shows reactivity with Human samples.
Tested Applications | WB, IHC, ELISA Application Description |
Tested Reactivity | Human |
Immunogen | ATP5D fusion protein Ag7016 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit |
Synonyms | ATP5D, F ATPase delta subunit |
Calculated Molecular Weight | 17 kDa |
Observed Molecular Weight | 15-17 kDa |
GenBank Accession Number | BC002389 |
Gene Symbol | ATP5D |
Gene ID (NCBI) | 513 |
RRID | AB_2882027 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P30049 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The mitochondrial F1Fo-ATP synthase complex uses energy derived from a proton gradient to synthesize ATP. The ATP5D gene encodes the delta subunit of the mitochondrial ATP synthase F1 complex and belongs to the ATPase epsilon chain family. This gene encode the full length protein of 17 kDa with a transit peptide of 22 amino acid.
实验方案
Product Specific Protocols | |
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WB protocol for ATP5D antibody 66673-1-Ig | Download protocol |
IHC protocol for ATP5D antibody 66673-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |