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ATPB Monoclonal antibody

ATPB Monoclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse , Rat and More (1)

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A11B9

Cat No : 66600-1-Ig

Print datasheet

Synonyms

ATP5B, ATPB, ATPMB, ATPSB, Cell and organelle markers, Mitochondria Marker, Mitochondrial Marker, Mitochondrion Marker

验证数据展示

  • WB analysis using 66600-1-Ig

    WB analysis using 66600-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66600-1-Ig (ATPB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 66600-1-Ig

    IHC staining of human liver using 66600-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66600-1-Ig (ATPB antibody) at dilution of 1:500 (under 10x lens).

    Ready-to-use IHC kits available with ATP5B antibody included
  • IHC staining of human liver using 66600-1-Ig

    IHC staining of human liver using 66600-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66600-1-Ig (ATPB antibody) at dilution of 1:500 (under 40x lens).

    Ready-to-use IHC kits available with ATP5B antibody included
  • IF Staining of HepG2 using 66600-1-Ig

    IF Staining of HepG2 using 66600-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATPB antibody (66600-1-Ig, Clone: 1A11B9 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSCT6 cells, Raw 264.7 cells, NIH/3T3 cells
Positive IHC detected inhuman liver tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ATP5B Ready-To-Use IHC Kit. ATP5B primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66600-1-Ig targets ATPB in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse , Rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human, Mouse , Rat
Cited Reactivityrat
Immunogen ATPB fusion protein Ag11177 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
Synonyms ATP5B, ATPB, ATPMB, ATPSB, Cell and organelle markers, Mitochondria Marker, Mitochondrial Marker, Mitochondrion Marker
Calculated Molecular Weight 529 aa, 57 kDa
Observed Molecular Weight45-57 kDa
GenBank Accession NumberBC016512
Gene Symbol ATP5B
Gene ID (NCBI) 506
RRIDAB_2881960
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP06576
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP5B, also named as ATPMB and ATPSB, belongs to the ATPase alpha/beta chains family. It is a high affinity HDL receptor for apolipoprotein A1. ATP5B is a subunit of mitochondrial ATP synthase (F1F0 ATP synthase or Complex V ). ATP5B has a calculated molecular mass of 57 kDa and ~5 kDa transit peptide can be removed in the mature form.

实验方案

Product Specific Protocols
WB protocol for ATPB antibody 66600-1-IgDownload protocol
IHC protocol for ATPB antibody 66600-1-IgDownload protocol
IF protocol for ATPB antibody 66600-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Cell Biol Int

The effect of all-trans retinoic acid on the mitochondrial function and survival of cardiomyoblasts exposed to local photodamage

Authors - Simona Kurekova
View Article